Geng Ziyao, Xu Fan, Liu Ying, Qiao Aike, Du Tianming
Beijing International Science and Technology Cooperation Base for Intelligent Physiological Measurement and Clinical Transformation, Department of Biomedical Engineering, College of Chemistry and Life Sciences, Beijing University of Technology, Beijing 100124, China.
J Funct Biomater. 2024 Nov 22;15(12):356. doi: 10.3390/jfb15120356.
The cross-linking process of collagen is one of the more important ways to improve the mineralization ability of collagen. However, the regulatory effect of dynamic cross-linking on biomineralization in vitro remains unclear. Dynamic-cross-linked mineralized collagen under different cross-linking processes, according to the process of cross-linking and mineralization of natural bone, was prepared in this study. Mineralization was performed for 12 h at 4, 8, and 12 h of collagen cross-linking. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed the characteristics of dynamic-cross-linked mineralization in terms of morphological transformation and distribution. Fourier transform infrared spectroscopy (FTIR) analysis showed the crystallinity characteristics of the hydroxyapatite (HA) crystal formation. Pre-cross-linked dynamic-cross-linked mineralization refers to the process of cross-linking for a period of time and then side cross-linked mineralization. The mineral content, enzyme stability, and mechanical properties of mineralized collagen were improved through a dynamic cross-linking process of pre-cross-linking. The swelling performance was reduced through the dynamic cross-linking process of pre-cross-linking. This study suggests that the dynamic cross-linking process through pre-cross-linking could make it easier for minerals to permeate and deposit between collagen fibers, improve mineralization efficiency, and, thus, enhance the mechanical strength of biomineralization. This study can provide new ideas and a theoretical basis for designing mineralized collagen scaffolds with better bone repair ability.
胶原蛋白的交联过程是提高胶原蛋白矿化能力的重要途径之一。然而，动态交联对体外生物矿化的调控作用仍不清楚。本研究根据天然骨的交联和矿化过程，制备了不同交联过程下的动态交联矿化胶原蛋白。在胶原蛋白交联4、8和12小时时进行12小时的矿化。扫描电子显微镜（SEM）和透射电子显微镜（TEM）从形态转变和分布方面展示了动态交联矿化的特征。傅里叶变换红外光谱（FTIR）分析显示了羟基磷灰石（HA）晶体形成的结晶度特征。预交联动态交联矿化是指先交联一段时间然后进行侧交联矿化的过程。通过预交联的动态交联过程提高了矿化胶原蛋白的矿物质含量、酶稳定性和力学性能。通过预交联的动态交联过程降低了膨胀性能。本研究表明，通过预交联的动态交联过程可以使矿物质更容易渗透并沉积在胶原纤维之间，提高矿化效率，从而增强生物矿化的机械强度。本研究可为设计具有更好骨修复能力的矿化胶原蛋白支架提供新思路和理论依据。
