Alberto Vazquez Maryen, Saa Luis Rodrigo, Valiño Elaine, Torta Livio, Laudicina Vito Armando
Institute of Animal Science (ICA), San José de Las Lajas 32700, Cuba.
Laboratorio de Sanidad Animal y Zoonosis, Departmento de Ciencias Biologicas y Agropecuria, Escuela de Ingeniería Agropecuaria, Universidad Técnica Particular de Loja, Loja 1101608, Ecuador.
J Fungi (Basel). 2024 Nov 21;10(12):807. doi: 10.3390/jof10120807.
The complex structure of the plant cell wall makes it difficult to use the biomass produced by biosynthesis. For this reason, the search for new strains of microorganisms capable of efficiently degrading fiber is a topic of interest. For these reasons, the present study aimed to evaluate both the microbiological and enzymatic characteristics of the fungus L7strain. For this, its growth in different culture media was evaluated. Wheat straw mineralization was evaluated by gas chromatography assisted by infrared spectroscopy. The production of endo- and exoglucanase, laccase, and peroxidase enzymes in submerged solid fermentation of wheat and sugarcane bagasse were characterized. The strain efficiently mineralized raw wheat straw, showing a significant decrease in signals associated with cellulose, hemicellulose, and lignin in the infrared spectra. High enzyme productions were achieved in submerged solid fermentation of both substrates, highlighting the high production of endoglucanases in sugarcane bagasse (2.87 IU/mL) and laccases in wheat (1.64 IU/mL). It is concluded that L7 is an ascomycete with a versatile enzyme production that allows it to exhaustively degrade complex fibers such as raw wheat straw and sugar cane bagasse, making it a microorganism with great potential in the bioconversion of plant biomass.
植物细胞壁的复杂结构使得利用生物合成产生的生物质变得困难。因此，寻找能够有效降解纤维的新型微生物菌株是一个备受关注的课题。基于这些原因，本研究旨在评估L7菌株的微生物学和酶学特性。为此，评估了其在不同培养基中的生长情况。通过气相色谱-红外光谱联用技术评估了小麦秸秆的矿化情况。对小麦和甘蔗渣的固态发酵中内切葡聚糖酶、外切葡聚糖酶、漆酶和过氧化物酶的产生进行了表征。该菌株能有效地使未加工的小麦秸秆矿化，红外光谱显示与纤维素、半纤维素和木质素相关的信号显著降低。在两种底物的固态发酵中均实现了高酶产量，突出表现在甘蔗渣中内切葡聚糖酶的高产量（2.87 IU/mL）和小麦中漆酶的高产量（1.64 IU/mL）。结论是，L7是一种具有多种酶产生能力的子囊菌，能够彻底降解如未加工的小麦秸秆和甘蔗渣等复杂纤维，使其成为植物生物质生物转化中具有巨大潜力的微生物。
