Lei Yu, Lyu Aiyuan, Pan Mengjuan, Shi Qingxia, Xu Haowan, Li Dong, Deng Mengsheng
School of Biological Engineering, Sichuan University of Science & Engineering, Yibin 644000, China.
J Fungi (Basel). 2024 Dec 3;10(12):837. doi: 10.3390/jof10120837.
This study examined the efficacy and mechanisms of action of the antimicrobial peptide BP15 and its lipopeptides, HBP15 and LBP15, against , the primary causative agent of green mold in citrus fruits. The findings revealed that all three antimicrobial peptides markedly inhibited the spore germination and mycelial growth of , with minimum inhibitory concentrations (MICs) of 3.12 μM for BP15, HBP15, and LBP15. The peptides induced morphological alterations in hyphae and elevated intracellular Sytox Green (SG) fluorescence signals, which is indicative of increased cell membrane permeability and disruption. This membrane damage was further supported by the heightened extracellular conductivity and the release of intracellular nucleic acid and protein. A gel retardation assay demonstrated that the peptides showed significant DNA binding and retardation effects. Furthermore, the peptides exhibited significantly lower hemolytic activity ( < 0.05) compared to commercial prochloraz in normal mammalian erythrocytes (sheep erythrocytes) at the tested concentrations. Therefore, BP15 and its lipopeptides, HBP15 and LBP15, show potential as effective agents for preventing green mold in citrus fruits.
本研究考察了抗菌肽BP15及其脂肽HBP15和LBP15对柑橘类水果绿霉病主要病原菌的作用效果及作用机制。研究结果显示，这三种抗菌肽均能显著抑制该病原菌的孢子萌发和菌丝生长，BP15、HBP15和LBP15的最小抑菌浓度（MIC）均为3.12 μM。这些肽可诱导菌丝形态改变，并提高细胞内Sytox Green（SG）荧光信号，这表明细胞膜通透性增加且受到破坏。细胞外电导率升高以及细胞内核酸和蛋白质的释放进一步证明了这种膜损伤。凝胶阻滞试验表明，这些肽具有显著的DNA结合和阻滞作用。此外，在测试浓度下，与商业杀菌剂咪鲜胺相比，这些肽在正常哺乳动物红细胞（绵羊红细胞）中的溶血活性显著更低（<0.05）。因此，BP15及其脂肽HBP15和LBP15具有作为预防柑橘类水果绿霉病有效药剂的潜力。
