Evaluation of Lipid Changes During the Drying Process of by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS/MS)-Based Lipidomics Technique.

Comprehensive analysis of the lipid content in samples is essential for optimizing their effective use. Understanding the lipid profile can significantly enhance the application of this valuable fungus across various fields, including nutrition and medicine. However, to date, there is limited knowledge regarding the effects of different drying methods on the quality of lipids present in . In this study, we employed a broadly targeted lipidomic strategy to conduct a comprehensive analysis of the lipid composition in subjected to various drying methods. A comprehensive analysis identified a total of 765 distinct lipid species from fresh (FC), vacuum-freeze-dried (VG), oven-dried (OG), and air-dried (AG). Among these, glycerophospholipids (GP) were the most abundant, followed by glycerides (GL) and sphingolipids (SP). In this study, a total of 659 lipids demonstrated statistically significant differences, as indicated by a -value () < 1. Among these lipids, triglycerides (TG) exhibited the highest concentration, followed by several others, including ceramide-ascorbic acid (Cer-AS), phosphatidylethanolamine (PE), lysophosphatidylcholine (LPC), and phosphatidylserine (PS). OG was the fastest drying method; however, PCA and OPLS-DA analyses indicated that the most significant changes in the lipids of were observed under the OG method. Specifically, 517 differentially accumulated lipids were significantly down-regulated, while only 10 lipids were significantly up-regulated. This disparity may be attributed to the degradation and oxidation of lipids. The metabolic pathways of glycerolipid, glycerophospholipid, and cholesterol are critical during the drying process of . This study provides valuable insights that can enhance quality control and offer guidelines for the appropriate storage of this medicinal fungus.