Skóra Magdalena, Rosam Katharina, Namysł Magdalena, Sepioło Anna, Gajda Mateusz, Jędras Justyna, Krzyściak Paweł, Zorska Joanna, Wordliczek Jerzy, Heczko Piotr B, Würzner Reinhard, Lackner Michaela, Wójkowska-Mach Jadwiga
Chair of Microbiology, Faculty of Medicine, Jagiellonian University Medical College, Czysta 18 Street, 31-121 Krakow, Poland.
Institute for Hygiene and Medical Microbiology, Medical University of Innsbruck, Schöpfstraße 41, 6020 Innsbruck, Austria.
J Fungi (Basel). 2024 Dec 13;10(12):864. doi: 10.3390/jof10120864.
Invasive candidiasis is a predominant mycosis in hospitalized patients, and is the species most often responsible for this infection. Most candidiasis cases originate from endogenous mycobiota; therefore, strains can easily be transferred among hospital patients and personnel. The aim of this study was to assess the possible horizontal transmission of in patients with severe COVID-19 infection requiring hospitalization in the intensive care unit.
In total, 59 strains from 36 patients were collected from blood and lower-respiratory samples. The strains were genotyped using the RAPD method with the OPA-18 primer (5'-AGCTGACCGT-3'). Antifungal susceptibility testing was performed for amphotericin B (AMB), fluconazole (FCZ), voriconazole (VCZ), and anidulafungin (ANF) using the EUCAST method.
strains were separated into 13 different groups according to their RAPD pattern. Two predominant clonal clusters of 17 strains isolated from 12 patients and 12 strains from 7 patients were identified, followed by clusters with 6, 4, and 8 strains isolated from 5, 4, and 3 patients, respectively. strains were sensitive to AMB, FCZ, VCZ, and ANF, and antifungal susceptibility profiles were similar in all genetic clusters.
Our study indicates that strains can spread horizontally. The routes of transmission for strains in the ward have not been explained due to there being insufficient data. The transmission could have been caused by the unintentional spread of pathogens by medical personnel.
侵袭性念珠菌病是住院患者中主要的真菌病，且是最常导致这种感染的菌种。大多数念珠菌病病例源自内源性真菌群；因此，菌株可轻易在医院患者和医护人员之间传播。本研究的目的是评估在需要入住重症监护病房的重症 COVID-19 感染患者中，[具体菌种未提及]可能的水平传播情况。
总共从 36 名患者的血液和下呼吸道样本中收集了 59 株[具体菌种未提及]菌株。使用 OPA-18 引物（5'-AGCTGACCGT-3'）通过随机扩增多态性 DNA（RAPD）方法对菌株进行基因分型。使用欧洲抗菌药物敏感性试验委员会（EUCAST）方法对两性霉素 B（AMB）、氟康唑（FCZ）、伏立康唑（VCZ）和阿尼芬净（ANF）进行抗真菌药敏试验。
根据 RAPD 模式，[具体菌种未提及]菌株被分为 13 个不同的组。鉴定出两个主要的克隆簇，分别来自 12 名患者的 17 株菌株和来自 7 名患者的 12 株菌株，随后是分别来自 5 名、4 名和 3 名患者的 6 株、4 株和 8 株菌株的簇。[具体菌种未提及]菌株对 AMB、FCZ、VCZ 和 ANF 敏感，并且在所有基因簇中抗真菌药敏谱相似。
我们的研究表明[具体菌种未提及]菌株可水平传播。由于数据不足，病房中[具体菌种未提及]菌株的传播途径尚未得到解释。传播可能是由医护人员无意传播病原体所致。
