Malysheva Svetlana V, Guillaume Benoît, Vanhee Céline, Masquelier Julien
Toxins, Organic Contaminants and Additives, Physical and Chemical Health Risks, Sciensano, Leuvensesteenweg 17, 3080 Tervuren, Belgium.
Medicines and Health Products, Physical and Chemical Health Risks, Sciensano, Juliette Wytsmanstraat 14, 1050 Brussels, Belgium.
Toxins (Basel). 2024 Nov 23;16(12):505. doi: 10.3390/toxins16120505.
Hydroxyanthracene derivatives (HADs) are plant substances produced by a variety of plant species, including different , , and species and These plants are often used in food supplements to improve bowel function. However, recently, the European Commission prohibited a number of HADs due to toxicological concerns. These HADs included aloin (aloin A and aloin B), aloe-emodin, emodin, and danthron. Most of the currently available analytical methods are restricted to the analysis of only these compounds and do not include other HADs. In this view, a multi-analyte method could be useful for both regulatory analysis and dietary intake studies. To this end, such a method, employing liquid chromatography-tandem mass spectrometry and targeting 16 different HADs, was developed and validated in this study. Limits of quantification were in the range from 0.025 mg kg to 1 mg kg. The recovery of the method was within the acceptable range of 80% to 120%, with the exception of physcion. Repeatability varied from 0.5% to 11.6%, and the range for within-laboratory reproducibility was from 3.4% to 16.3%. The expanded measurement uncertainty was below 50% for all HADs. Subsequently, 24 commercial samples of food supplements and herbal infusions sourced in Belgium were analyzed. The results indicated that although the industry put a great effort into minimizing the amount of aloin and danthron present in food supplements, more than half of the products still exceeded the maximum tolerated levels suggested for aloe-emodin and emodin.
羟基蒽衍生物（HADs）是多种植物产生的植物物质，包括不同的[此处原文缺失植物种类名称]、[此处原文缺失植物种类名称]和[此处原文缺失植物种类名称]物种以及[此处原文缺失植物种类名称]。这些植物常用于食品补充剂中以改善肠道功能。然而，最近欧盟委员会因毒理学问题禁止了多种HADs。这些HADs包括芦荟苷（芦荟苷A和芦荟苷B）、芦荟大黄素、大黄素和丹蒽醌。目前大多数可用的分析方法仅限于分析这些化合物，不包括其他HADs。鉴于此，一种多分析物方法对于监管分析和膳食摄入研究可能会很有用。为此，本研究开发并验证了一种采用液相色谱 - 串联质谱法、针对16种不同HADs的方法。定量限在0.025 mg/kg至1 mg/kg范围内。该方法的回收率在可接受的80%至120%范围内，但大黄素甲醚除外。重复性在0.5%至11.6%之间变化，实验室内再现性范围为3.4%至16.3%。所有HADs的扩展测量不确定度均低于50%。随后，对来自比利时的24个商业食品补充剂和草药浸液样品进行了分析。结果表明，尽管该行业在尽量减少食品补充剂中芦荟苷和丹蒽醌的含量方面付出了巨大努力，但仍有超过一半的产品超过了芦荟大黄素和大黄素建议的最大耐受水平。
