Galli Corrado L, Cinelli Serena, Ciliutti Paola, Melzi Gloria, Marinovich Marina
Department of Pharmacological and Biomolecular Sciences (DiSFeB) Section of Toxicology and Risk Assessment, University of Milan, Milan, Italy.
European Research Biology Center, ERBC, Pomezia, Rome, Italy.
Toxicol Rep. 2021 Aug 3;8:1471-1474. doi: 10.1016/j.toxrep.2021.07.023. eCollection 2021.
Mill is widely used as a traditional herbal medicine for the treatment of a broad spectrum of illnesses given its laxative, anti-inflammatory, bitter tonic, anti-oxidant, antimicrobial and anti-cancer properties. Using the alkaline comet assay in animals (OECD 489), this study investigated the potential genotoxicity of dried juice at dose levels of 500, 1000, and 2000 mg/kg/day in mice. showed no genotoxic activity in preparations of single cells from the colon of the treated Hsd:ICR (CD-1) male mice. No statistically significant increase in DNA migration over the negative control was observed by analysis of variance for both comet parameters, tail moment and tail intensity, apart from the positive control ethyl methanesulphonate that induced clear and statistically significant increases in DNA migration parameters over the concurrent controls. The new reported scientific evidence unequivocally demonstrates that dried juice containing hydroxyanthracene derivatives does not induce DNA damage in preparations of single cells from colon in comet genotoxicity studies. This suggests that the hyperplastic changes and mucosal hyperplasia observed after long-term administration of non-decolourised whole leaf extract may be attributed to an epigenetic effect of the material under investigation.
鉴于其具有通便、抗炎、苦味滋补、抗氧化、抗菌和抗癌特性，番泻叶作为一种传统草药被广泛用于治疗多种疾病。本研究采用动物碱性彗星试验（经合组织489），在小鼠中以500、1000和2000毫克/千克/天的剂量水平研究了干汁的潜在遗传毒性。在经处理的Hsd:ICR（CD-1）雄性小鼠结肠单细胞制剂中未显示出遗传毒性活性。通过对彗星试验的两个参数（尾矩和尾强度）进行方差分析，除了阳性对照甲磺酸乙酯在同时期对照中诱导DNA迁移参数出现明显且具有统计学意义的增加外，未观察到DNA迁移相对于阴性对照有统计学意义的增加。新报道的科学证据明确表明，在彗星遗传毒性研究中，含有羟基蒽衍生物的干汁不会在结肠单细胞制剂中诱导DNA损伤。这表明长期给予未脱色全叶提取物后观察到的增生性变化和黏膜增生可能归因于所研究物质的表观遗传效应。
