García Marina, Martínez-Flórez Icíar, Solano-Gallego Laia, García Nuria, Ordeix Laura
Fundació Hospital Clínic Veterinari, Universitat Autὸnoma de Barcelona, 08193 Bellaterra, Spain.
Departament de Medicina i Cirurgia Animals, Universitat Autὸnoma de Barcelona, 08193 Bellaterra, Spain.
Vet Sci. 2024 Dec 7;11(12):634. doi: 10.3390/vetsci11120634.
This study aimed to evaluate differences in the production of reactive oxygen species (ROS) by peripheral blood neutrophils in healthy dogs, dogs with superficial pyoderma, and dogs with sterile neutrophilic dermatitis using the nitroblue tetrazolium (NBT) reduction test. Additionally, the study assessed the potential of the NBT reduction test as a diagnostic tool to differentiate between these clinical conditions. A total of 28 dogs were divided into three groups: healthy (n = 10), superficial pyoderma (n = 10), and sterile neutrophilic dermatitis (n = 8). The results showed significantly higher neutrophil activation in dogs with sterile neutrophilic dermatitis (19 ± 10%) compared with healthy dogs (7.3 ± 5%) ( < 0.01) and dogs with superficial pyoderma (10 ± 2.8%) ( < 0.05). Dogs with sterile neutrophilic dermatitis and a high positive result for spp. antibodies (n = 3) exhibited even higher NBT reduction rates (30 ± 4.6%) compared with seronegative dogs with sterile neutrophilic dermatitis (n = 5) (13 ± 5.3%) ( < 0.05). These findings suggest that the NBT reduction test could be useful for detecting systemic neutrophil activation in sterile neutrophilic dermatitis, especially when associated with infection. However, no significant differences were observed between healthy dogs and those with superficial pyoderma, indicating that the test may not be effective in detecting systemic neutrophil activation in superficial bacterial infections.
本研究旨在使用硝基蓝四氮唑（NBT）还原试验评估健康犬、浅表脓皮病犬和无菌性嗜中性皮炎犬外周血中性粒细胞产生活性氧（ROS）的差异。此外，该研究评估了NBT还原试验作为区分这些临床病症的诊断工具的潜力。总共28只犬被分为三组：健康组（n = 10）、浅表脓皮病组（n = 10）和无菌性嗜中性皮炎组（n = 8）。结果显示，与健康犬（7.3±5%）（P<0.01）和浅表脓皮病犬（10±2.8%）（P<0.05）相比，无菌性嗜中性皮炎犬的中性粒细胞活化程度显著更高（19±10%）。与无菌性嗜中性皮炎血清阴性犬（n = 5）（13±5.3%）相比，无菌性嗜中性皮炎且对某些菌种抗体检测呈高阳性结果的犬（n = 3）表现出更高的NBT还原率（30±4.6%）（P<0.05）。这些发现表明，NBT还原试验可能有助于检测无菌性嗜中性皮炎中的全身中性粒细胞活化，尤其是与某些感染相关时。然而，在健康犬和浅表脓皮病犬之间未观察到显著差异，这表明该试验可能无法有效检测浅表细菌感染中的全身中性粒细胞活化。
