Li Guiyin, Wu Guangxiong, Huang Qing, Dong Shuaikang, Zhou Yu, Lu Mei, Liang Jintao, Zhou Xueqing, Zhou Zhide
College of Chemistry, Guangdong University of Petrochemical Technology, Guandu Road, Maoming, Guangdong, 525000, People's Republic of China.
School of Life and Environmental Sciences, School of Intellectual Property, Guilin University of Electronic Technology, Guilin, Guangxi, 541004, People's Republic of China.
Mikrochim Acta. 2024 Dec 27;192(1):35. doi: 10.1007/s00604-024-06909-w.
A novel carbon-based light-addressable potentiometric aptasensor (C-LAPS) was constructed for detection low-density lipoprotein (LDL) in serum. Carboxylated TiC MXene @reduced graphene oxide (C-MXene@rGO) was used as interface and o-phenylenediamine functionalized nitrogen-doped graphene quantum dots (OPD@NGQDs) as the photoelectric conversion element. The photosensitive layers composed of OPD@NGQDs/C-MXene@rGO exhibit superior photoelectric conversion efficiency and excellent biocompatibility, which contribute to an improved response signal. When LDL reacts with the LDL aptamer (LDL) immobilized on the photosensitive layers to form LDL-LDL complexes, the reaction process can induce the modification of the surface potential in the photosensitive layer, leading to potential shift observed through the I-V curves. The experimental conditions were successfully optimized with few planned tests by applying the Box-Behnken design and response surface methodology aspects of the Design-Expert software. Under the optimal condition, the potential shift had a linear relationship with concentrations of LDL from 0.02 to 0.30 μg/mL. The limit of detection (LOD) was 5.88 ng/mL (S/N = 3) and the sensitivity was 315.20 mV/μg·mL. In addition, the LDL C-LAPS demonstrated excellent specificity, reproducibility, and stability in detecting LDL. The sensor performed well in quantifying LDL in real samples. Therefore, the LDL C-LAPS has the potential for clinical applications.
构建了一种新型的基于碳的光寻址电位适体传感器（C-LAPS）用于检测血清中的低密度脂蛋白（LDL）。羧基化TiC MXene@还原氧化石墨烯（C-MXene@rGO）用作界面，邻苯二胺功能化的氮掺杂石墨烯量子点（OPD@NGQDs）用作光电转换元件。由OPD@NGQDs/C-MXene@rGO组成的光敏层表现出优异的光电转换效率和良好的生物相容性，这有助于提高响应信号。当LDL与固定在光敏层上的LDL适配体（LDL）反应形成LDL-LDL复合物时，反应过程会引起光敏层表面电位的改变，从而通过I-V曲线观察到电位变化。通过应用Design-Expert软件的Box-Behnken设计和响应面方法，仅通过少量计划测试就成功优化了实验条件。在最佳条件下，电位变化与0.02至0.30μg/mL的LDL浓度呈线性关系。检测限（LOD）为5.88 ng/mL（S/N = 3），灵敏度为315.20 mV/μg·mL。此外，LDL C-LAPS在检测LDL时表现出优异的特异性、重现性和稳定性。该传感器在实际样品中定量LDL方面表现良好。因此，LDL C-LAPS具有临床应用潜力。
