Li Bin, Wang Jinjin, Ge Jianlong, Liao Meijie, Rong Xiaojun, Wang Jinyan, Wang Yingeng, Zhang Zheng, Wang Chunyuan, Yu Yongxiang
Key Laboratory of Sustainable and Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, PR China.
Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, PR China.
PLoS One. 2024 Dec 27;19(12):e0315780. doi: 10.1371/journal.pone.0315780. eCollection 2024.
In order to study the optimal use of Lactobacillus plantarum in sea cucumber (Apostichopus japonicus), 49 days feeding trial was conducted to determine the influence of immersion bathing in different concentrations of Lactobacillus plantarum CLY-05 on body weight gain rate and non-specific immune activities. The potential effect of CLY-05 on gut microbiota was also analyzed during the immersion bathing at the optimum concentration. The results showed that the body weight growth rate of all bathing groups was higher than that of control. The highest specific growth rate (4.58%) and weight gain rate (25.35%) was achieved at the bacterial concentration of 1×103 CFU/mL. The activities of non-specific immune enzymes (ACP, AKP, SOD and LZM) of all bathing groups increased after immersion bathing, and the enzyme activities of groups bathed with the bacterium at 1×103 and 1×104 CFU/mL reached the highest. Therefore, 1×103 CFU/mL was considered as the optimum concentration of L. plantarum CLY-05 for A. japonicus pond culture. The results of gut microbiota analysis showed that the gut microbiota changed with the addition of L. plantarum CLY-05, and the richness and diversity of the gut microbiota peaked on day 14 and day 21, respectively. The correlation analysis revealed that the non-specific immune enzyme activities were significantly correlated to some gut bacteria (in the phyla Proteobacteria, Firmicutes) after immersion bathing in L. plantarum CLY-05. These findings provide the theoretical foundation for probiotic application in sea cucumber farming.
为研究植物乳杆菌在海参（刺参）中的最佳使用方法，进行了为期49天的投喂试验，以确定不同浓度的植物乳杆菌CLY-05浸浴对海参体重增加率和非特异性免疫活性的影响。在以最佳浓度浸浴期间，还分析了CLY-05对肠道微生物群的潜在影响。结果表明，所有浸浴组的体重增长率均高于对照组。在细菌浓度为1×10³ CFU/mL时，获得了最高的特定生长率（4.58%）和体重增加率（25.35%）。所有浸浴组的非特异性免疫酶（ACP、AKP、SOD和LZM）活性在浸浴后均有所增加，以1×10³和1×10⁴ CFU/mL细菌浸浴的组酶活性达到最高。因此，1×10³ CFU/mL被认为是植物乳杆菌CLY-05用于刺参池塘养殖的最佳浓度。肠道微生物群分析结果表明，肠道微生物群随植物乳杆菌CLY-05的添加而变化，肠道微生物群的丰富度和多样性分别在第14天和第21天达到峰值。相关性分析表明，在植物乳杆菌CLY-05浸浴后，非特异性免疫酶活性与一些肠道细菌（变形菌门、厚壁菌门）显著相关。这些发现为益生菌在海参养殖中的应用提供了理论基础。
