Ponticelli Maria, Esposito Germana, Labanca Fabiana, Scognamiglio Paolo, Sinisgalli Chiara, Milella Luigi, Faraone Immacolata, Costantino Valeria
Department of Health Science, Universitá degli Studi della Basilicata, Via dell'Ateneo Lucano 10, 85100 Potenza, Italy; Department of Biochemical Pharmacology & Drug Design, Institute of Molecular Biology "Roumen Tsanev", Bulgarian Academy of Sciences (BAS), Acad. G. Bonchev Str., bl. 21, 1113 Sofia, Bulgaria.
The Blue Chemistry Lab Group, Department of Pharmacy, Università degli Studi di Napoli Federico II, Napoli, Italy.
Food Chem. 2025 Mar 30;469:142573. doi: 10.1016/j.foodchem.2024.142573. Epub 2024 Dec 18.
Grape pomace (GP), a by-product of the wine supply chain process, contains bioactive molecules with known healthy properties. This study examines the impact of different extraction techniques on three GPs of Aglianico cultivar [Cantine del Notaio, Barile, and Torrecuso]. Five eco-friendly extractive techniques [maceration (MAC), digestion (DIG), accelerated solvent extraction (ASE), microwaves (MW), and ultrasound (US)] were used with 50 % ethanol/water as solvent. Spectrophotometric assays showed that DIG and ASE extracts had the highest antioxidant activity and specialized metabolite content. Using the HRMS/MS-based molecular networking, DIG and ASE extract metabolome profiles were analyzed, identifying unknown compounds and known ones with validated antioxidant and chemopreventive effects. In vitro cell-based assay on HepG2 cells demonstrated that Barile GP DIG extract has the highest anti-proliferative activity. Hence, this work provides insight into the potential application of Barile GP DIG extract as a rich source of specialized metabolites with antioxidant and anti-proliferative activity.
葡萄皮渣（GP）是葡萄酒供应链过程中的一种副产品，含有具有已知健康特性的生物活性分子。本研究考察了不同提取技术对三种阿利安尼科品种[诺泰奥酒庄、巴里勒和托雷库索]葡萄皮渣的影响。使用了五种环保提取技术[浸渍法（MAC）、消化法（DIG）、加速溶剂萃取法（ASE）、微波法（MW）和超声法（US）]，以50%乙醇/水作为溶剂。分光光度法测定表明，DIG和ASE提取物具有最高的抗氧化活性和特殊代谢物含量。利用基于高分辨质谱/质谱的分子网络技术，对DIG和ASE提取物的代谢组学图谱进行了分析，鉴定出未知化合物以及具有已证实抗氧化和化学预防作用的已知化合物。对HepG2细胞进行的体外细胞实验表明，巴里勒葡萄皮渣DIG提取物具有最高的抗增殖活性。因此，本研究为巴里勒葡萄皮渣DIG提取物作为具有抗氧化和抗增殖活性的特殊代谢物丰富来源的潜在应用提供了见解。
