Chen Jialuo, Li Zhipeng, Chen Xinpeng, Sun Yurong, Cheng Jin, Li Aijing, Lu Shenzhou, Xing Tieling
National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou 215123, China.
ACS Biomater Sci Eng. 2025 Jan 13;11(1):343-353. doi: 10.1021/acsbiomaterials.4c01659. Epub 2024 Dec 27.
Adhesives have garnered significant interest recently due to their application in the field of biomedical applications. Nonetheless, developing adhesives that exhibit robust underwater adhesion and possess antimicrobial properties continues to pose a significant challenge. In this study, motivated by the adhesive mechanism observed in mussels in aquatic environments, dopamine (DA) was added to modify the silk fibroin (SF) solution. Subsequently, tea polyphenol (TP) was incorporated to form a sticky mixture, resulting in a biomimetic adhesive (TP-DA/SF). TP-DA/SF demonstrated rapid, robust, and indiscriminate adhesion to a wide array of substrates and even biological tissues (39 kPa). TP-DA/SF exhibits the ability to replicate the mussel adhesion mechanism of mussels underwater thanks to its biomimetic design. This characteristic provides the material with robust adhesion (40 kPa), notable reusability (at least 10 times), and long-lasting stability, especially in aquatic settings. It is worth noting that TP-DA/SF also demonstrated high adhesion in various water environments, even in solutions with a pH of 7.4 and buffered saline (PBS), which is one of the most widely used buffers in biochemistry research, offering salt-balancing and adjustable pH buffering capabilities. Meanwhile, TP-DA/SF exhibits excellent antibacterial and antioxidant properties due to its tea polyphenol content. After 15 days of wound closure in SD rats, the healing rate in the experimental group reached 93.4%, compared to 83.9% in the control group. Thus, the TP-DA/SF adhesive holds promising potential for biomedical applications, including sutureless wound closure and tissue adhesion.
由于粘合剂在生物医学应用领域的应用，近年来它们引起了极大的关注。尽管如此，开发具有强大水下粘附力并具备抗菌性能的粘合剂仍然是一项重大挑战。在本研究中，受水生环境中贻贝粘附机制的启发，添加多巴胺（DA）以修饰丝素蛋白（SF）溶液。随后，加入茶多酚（TP）形成粘性混合物，从而得到一种仿生粘合剂（TP-DA/SF）。TP-DA/SF对多种基材甚至生物组织都表现出快速、强大且无选择性的粘附力（39千帕）。由于其仿生设计，TP-DA/SF能够在水下复制贻贝的粘附机制。这一特性赋予该材料强大的粘附力（40千帕）、显著的可重复使用性（至少10次）以及持久的稳定性，尤其是在水生环境中。值得注意的是，TP-DA/SF在各种水环境中也表现出高粘附力，即使在pH值为7.4的溶液和缓冲盐水（PBS）中也是如此，PBS是生物化学研究中使用最广泛的缓冲液之一，具有盐平衡和可调节的pH缓冲能力。同时，由于其茶多酚含量，TP-DA/SF表现出优异的抗菌和抗氧化性能。在SD大鼠伤口闭合15天后，实验组的愈合率达到93.4%，而对照组为83.9%。因此，TP-DA/SF粘合剂在包括无缝合伤口闭合和组织粘附在内的生物医学应用中具有广阔的前景。
