van Fürden Betina, Humburg Anne, Fuss Elisabeth
Heinrich-Heine-Universität Düsseldorf, Institut für Entwicklungs- und Molekularbiologie der Pflanzen, Universitätsstr.1, 40225, Düsseldorf, Germany.
Plant Cell Rep. 2005 Jul;24(5):312-7. doi: 10.1007/s00299-005-0954-8. Epub 2005 Apr 8.
The accumulation of podophyllotoxin (PTOX) and 6-methoxypodophyllotoxin (6MPTOX) was enhanced about twofold in the suspension culture of Linum album line 2-5 aH following the addition of methyl jasmonate (MeJas) to the cultivation medium, reaching 7.69+/-1.45 mg/g dry weight and 1.11+/-0.09 mg/g dry weight, respectively. There was no increase in 6MPTOX accumulation following the addition of MeJas to suspension cells of L. album line X4SF, whereas PTOX accumulation was enhanced about tenfold to 0.49+/-0.10 mg/g dry weight. Phenylalanine ammonia-lyase activity increased immediately after the addition of MeJas to a cell suspension culture of line X4SF, reaching a maximum between 4 h and 1 day after elicitation, while cinnamyl alcohol dehydrogenase activity and the lignin content of the cells were not affected.
在亚麻属品系2-5 aH的悬浮培养中，向培养基中添加茉莉酸甲酯（MeJas）后，鬼臼毒素（PTOX）和6-甲氧基鬼臼毒素（6MPTOX）的积累量提高了约两倍，分别达到7.69±1.45毫克/克干重和1.11±0.09毫克/克干重。向亚麻属品系X4SF的悬浮细胞中添加MeJas后，6MPTOX的积累量没有增加，而PTOX的积累量提高了约十倍，达到0.49±0.10毫克/克干重。向品系X4SF的细胞悬浮培养物中添加MeJas后，苯丙氨酸解氨酶活性立即增加，在诱导后4小时至1天达到最大值，而肉桂醇脱氢酶活性和细胞的木质素含量不受影响。
