Ebersole Jeffrey L, Kirakodu Sreenatha S, Zhang Xiaohua D, Dawson Dolph, Miller Craig S
Department of Biomedical Sciences, School of Dental Medicine, University of Nevada Las Vegas, Las Vegas, NV, 89131, USA.
Center for Oral Health Research, College of Dentistry, University of Kentucky, Lexington, KY, USA.
Sci Rep. 2024 Dec 28;14(1):30649. doi: 10.1038/s41598-024-77434-2.
Type 2 diabetes mellitus (T2DM) is associated with cellular abnormalities, tissue and organ dysfunctions, and periodontitis. This investigation examined the relationship between the oral microbiome and salivary biomarkers in T2DM patients with or without periodontitis. This cohort (35-80 years) included systemically healthy non-periodontitis (NP; n = 31), T2DM without periodontitis (DWoP; n = 32) and T2DM with periodontitis (DWP; n = 29). The oral microbiome [Operational Taxonomic Units (OTUs)] (16 s rRNA sequencing) and targeted host salivary biomarkers (immunoassays) were assessed. We identified 47 OTUs that were significantly different in abundance between NP samples and any disease subset or between disease subgroups. The most unique microbiome patterns were observed in the DWP group. Differences in genera/species abundance were also observed when T2DM patients were stratified by extent of periodontal inflammation and disease (i.e., generalized versus localized gingivitis/periodontitis). Salivary biomarkers showed significant elevations in MMP-8, MMP-9, resistin, IL-1β, IL-6, IFNα, and BAFF (THFSR13b) comparing generalized to localized periodontitis. Salivary analytes showed significant positive correlations with specific microbiome members, predominantly in DWP patients. Odds ratio analyses reinforced that a panel of biologic markers (IL-6, MMP-8) and bacteria (e.g., Bacteroidetes, Fusobacteria, Spirochaetes) discriminated the severity and extent of periodontal disease in this diabetic population.
2型糖尿病（T2DM）与细胞异常、组织和器官功能障碍以及牙周炎有关。本研究调查了有无牙周炎的T2DM患者口腔微生物群与唾液生物标志物之间的关系。该队列（35 - 80岁）包括全身健康的非牙周炎患者（NP；n = 31）、无牙周炎的T2DM患者（DWoP；n = 32）和有牙周炎的T2DM患者（DWP；n = 29）。评估了口腔微生物群[操作分类单元（OTU）]（16s rRNA测序）和靶向宿主唾液生物标志物（免疫测定）。我们鉴定出47个OTU，其在NP样本与任何疾病亚组之间或疾病亚组之间的丰度存在显著差异。在DWP组中观察到最独特的微生物群模式。当根据牙周炎症和疾病程度（即广泛性与局限性牙龈炎/牙周炎）对T2DM患者进行分层时，也观察到属/种丰度的差异。与局限性牙周炎相比，广泛性牙周炎患者的唾液生物标志物MMP - 8、MMP - 9、抵抗素、IL - 1β、IL - 6、IFNα和BAFF（THFSR13b）显著升高。唾液分析物与特定微生物群成员呈显著正相关，主要在DWP患者中。优势比分析强化了一组生物标志物（IL - 6、MMP - 8）和细菌（如拟杆菌门、梭杆菌门、螺旋体门）可区分该糖尿病群体中牙周疾病的严重程度和范围。
