Li Jianbo, Ryan Merrill, Dong Chongmei, Forrest Kerrie L, Hayden Matthew J, Singh Smriti, Wang Yuqi, Ahmed Nizam, McIntosh Robert A, Zhang Peng
Plant Breeding Institute, School of Life and Environmental Sciences, The University of Sydney, Cobbitty, NSW, 2570, Australia.
Department of Agriculture and Fisheries Queensland, Warwick, QLD, 4370, Australia.
Theor Appl Genet. 2024 Dec 27;138(1):15. doi: 10.1007/s00122-024-04807-6.
We analysed the chromosomal structures of two wheat-Thinopyrum intermedium addition lines Z4 and Z5 and resolved the linkage relationship between the leaf rust and stripe rust resistance genes in Z4. Wheat addition lines Z4 and Z5 carrying rust resistance genes from Thinopyrum intermedium (JJJJStSt, 2n = 6x = 42) together with three wheat lines involved in the production of these addition lines were analysed by rust response, 90K SNP genotyping, and molecular cytogenetic analysis. Seedling leaf rust (LR) responses to five diverse pathotypes indicated that the LR resistance gene(s) was located in translocation chromosome T3DS-3AS.3AL-7StS common to Z4 and Z5. The stripe rust (YR) resistance gene(s) was located in translocation chromosome T3AL-7StS.7StL, which is unique to Z4, based on the seedling YR responses to four diverse pathotypes. Backcross and selfed populations involving the addition lines and various wheat cultivars were studied to understand the inheritance of the alien resistance genes. Although inheritance studies indicated genetic linkage, the alien genes for resistance to leaf rust (LR) and stripe rust (YR) in Z4 were present in different wheat-Th. intermedium translocation chromosomes. We found that LR and YR were in pseudo-linkage, rather than true linkage.
我们分析了两个小麦-中间偃麦草附加系Z4和Z5的染色体结构，并解析了Z4中抗叶锈病和抗条锈病基因之间的连锁关系。通过锈病抗性反应、90K SNP基因分型和分子细胞遗传学分析，对携带来自中间偃麦草（JJJJStSt，2n = 6x = 42）抗锈病基因的小麦附加系Z4和Z5以及参与这些附加系培育的三个小麦品系进行了分析。对五种不同致病型的幼苗叶锈病（LR）反应表明，LR抗性基因位于Z4和Z5共有的易位染色体T3DS-3AS.3AL-7StS上。基于对四种不同致病型的幼苗条锈病（YR）反应，YR抗性基因位于Z4特有的易位染色体T3AL-7StS.7StL上。研究了涉及附加系和各种小麦品种的回交和自交群体，以了解外源抗性基因的遗传情况。尽管遗传研究表明存在遗传连锁，但Z4中抗叶锈病（LR）和抗条锈病（YR）的外源基因存在于不同的小麦-中间偃麦草易位染色体上。我们发现LR和YR是假连锁，而非真连锁。
