Crop Research Institute, Shandong Academy of Agricultural Sciences/Key Laboratory of Wheat Biology and Genetic Improvement in the North Huang and Huai River Valley, Ministry of Agriculture/National Engineering Laboratory for Wheat and Maize, Jinan, Shandong 250100, China.
Plant Breeding Institute, School of Life and Environmental Sciences, The University of Sydney, Sydney, New South Wales 2570, Australia.
Plant Dis. 2022 Sep;106(9):2447-2454. doi: 10.1094/PDIS-10-21-2274-RE. Epub 2022 Aug 11.
(JJJJStSt, 2 = 6 = 42), a wild relative of common wheat, possesses many desirable agronomic genes for wheat improvement. The production of wheat- introgression lines is a key step for transferring these beneficial genes into wheat. In this study, we characterized three wheat- introgression lines TA3681, TA5566, and TA5567 using non-denaturing fluorescence in situ hybridization, genomic in situ hybridization, PCR-based landmark unique gene, and intron targeting markers. Our results showed that TA3681 is a wheat- 1St disomic addition line, TA5566 is a wheat- non-Robertsonian translocation line carrying two pairs of 3A-7J translocation chromosomes, and that TA5567 is a wheat- non-Robertsonian translocation line carrying a pair of 3A-7J translocation chromosomes. We developed 13, 36, and 15 chromosome-specific markers for detecting the introgressed chromosomes in TA3681, TA5566, and TA5567, respectively. Stem rust assessment revealed that TA3681 exhibited a high level of seedling resistance to Chinese-prevalent f. sp. pathotypes, and both TA5566 and TA5567 were highly resistant to Australian . f. sp. pathotypes, indicating that chromosomes 1St and 7J might carry new stem rust resistance genes. Therefore, the new identified introgression lines may be useful for improving wheat stem rust resistance.
（JJJJStSt，2=6=42）是普通小麦的野生近缘种，具有许多改良小麦的理想农艺基因。生产小麦渐渗系是将这些有益基因转移到小麦中的关键步骤。在这项研究中，我们使用非变性荧光原位杂交、基因组原位杂交、基于 PCR 的标志性独特基因和内含子靶向标记物对三个小麦渐渗系 TA3681、TA5566 和 TA5567 进行了特征描述。我们的结果表明，TA3681 是一个小麦 1St 二体附加系，TA5566 是一个携带两对 3A-7J 易位染色体的小麦非罗伯逊易位系，而 TA5567 是一个携带一对 3A-7J 易位染色体的小麦非罗伯逊易位系。我们分别为 TA3681、TA5566 和 TA5567 开发了 13、36 和 15 个染色体特异性标记来检测导入的染色体。茎锈病评估表明，TA3681 对中国流行的 f. sp. 生理小种表现出高水平的幼苗抗性，而 TA5566 和 TA5567 对澳大利亚的. f. sp. 生理小种均表现出高度抗性，表明 1St 和 7J 染色体可能携带新的茎锈病抗性基因。因此，新鉴定的渐渗系可能有助于提高小麦的茎锈病抗性。
