Sunami Hiroshi, Shimizu Yusuke, Nakasone Hitoshi, Futenma Naoko, Denda Junko, Yokota Sayaka, Kishimoto Hidehiro, Makita Masashi, Nishikawa Yasutoshi
Faculty of Medicine, University of the Ryukyus, Nishihara-cho, Japan.
Graduate School of Medicine, University of the Ryukyus, Nishihara-cho, Japan.
Biomed Eng Online. 2024 Dec 27;23(1):133. doi: 10.1186/s12938-024-01324-x.
A biodegradable nonwoven fabric that can be used to extract adipose-derived stem cells (ADSCs) from adipose tissue slices was developed, which were cultured rapidly without enzymatic treatment. The extracted and cultured ADSCs remain on the nonwoven fabric and form a thick cell sheet. The aim was to use the thick cell sheet as a treatment by transplanting it into the living body. In addition, the expectation was that it will be possible to observe the cell sheet in the living body using X-ray computed tomography (CT) because the nonwoven fabric used to produce the cell sheet contains 50% (by weight) hydroxyapatite.
Thick cell sheets of ADSCs supported by two layers of nonwoven fabric were cut to size and transplanted into the cheeks of rats. No health damage was observed in the rats in which the cell sheets were implanted, except for one in which the surgery appeared to have failed. X-ray CT imaging showed that the fabric of the implanted cell sheet biodegraded over 12 weeks. Changes in the position, shape, and size of the cell sheet within the rat's body were tracked by X-ray CT. The thick cell sheets, which can be easily produced by simply seeding tissue slices, can be cut into appropriate shapes and transplanted safely, and it was confirmed that they slowly biodegraded when transplanted into the rats' bodies.
We demonstrated not only that the thick ADSC sheets can be transplanted successfully into animals, but also that the transplanted sheets can be observed in vivo by X-ray CT, which also allows changes in the ADSC sheets to be tracked. The results suggest that the biodegradable nonwoven fabric will be a useful transplantation device to ensure cell engraftment throughout the affected area, and facilitate monitoring of the transplant's subsequent status. We expect that this transplantation device will promote the development of regenerative therapy.
开发了一种可生物降解的无纺布，可用于从脂肪组织切片中提取脂肪来源干细胞（ADSCs），无需酶处理即可快速培养。提取并培养的ADSCs保留在无纺布上并形成厚细胞片。目的是通过将厚细胞片移植到活体中来将其用作一种治疗方法。此外，由于用于生产细胞片的无纺布含有50%（重量）的羟基磷灰石，期望能够使用X射线计算机断层扫描（CT）在活体中观察细胞片。
将由两层无纺布支撑的ADSCs厚细胞片裁剪成合适尺寸并移植到大鼠脸颊。除了一例手术似乎失败的大鼠外，植入细胞片的大鼠未观察到健康损害。X射线CT成像显示，植入细胞片的织物在12周内发生生物降解。通过X射线CT追踪大鼠体内细胞片的位置、形状和大小变化。厚细胞片可通过简单接种组织切片轻松制备，可裁剪成合适形状并安全移植，并且证实当移植到大鼠体内时它们会缓慢生物降解。
我们不仅证明了厚ADSC片可以成功移植到动物体内，还证明了移植的片可以通过X射线CT在体内观察到，这也允许追踪ADSC片的变化。结果表明，这种可生物降解的无纺布将是一种有用的移植装置，可确保细胞在整个受影响区域植入，并便于监测移植后的状态。我们期望这种移植装置将促进再生治疗的发展。
