Liang Jiahui, Yang Maoxia, Li Xinmei, Zhou Qianjin, Yang Guanjun, Lu Jianfei, Chen Jiong
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, School of Marine Sciences, Ningbo University, Ningbo, 315211, China; Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo, 315211, China; Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Ningbo, 315211, China.
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, School of Marine Sciences, Ningbo University, Ningbo, 315211, China; Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo, 315211, China; Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Ningbo, 315211, China.
Fish Shellfish Immunol. 2025 Feb;157:110099. doi: 10.1016/j.fsi.2024.110099. Epub 2024 Dec 26.
CyHV-2 is the pathogen of herpesvirus hematopoietic necrosis (HVHN), resulting in significant economic losses in crucian carp. Although multiple oral vaccines have been developed to prevent CyHV-2, they have not achieved ideal protective effects. To improve the protective effect of oral vaccine, a combination vaccine was conducted by mixing recombinant Saccharomyces cerevisiae displaying ORF132 or ORF25 on the cell surface in a 1:1 ratio. Oral immunization with this combined vaccine induced specific antibodies against ORF132 and ORF25 in fish serum, as well as increased the transcription levels of IgT, IgM, IL-1β, IFN-1, and TNF-α in the spleen, head-kidney, and hindgut. Importantly, the combined vaccine provided significant protection against CyHV-2 infection in crucian carp, resulting in a relative percent survival of 80.77 %. Additionally, it suppressed viral load and alleviated pathological damage of CyHV-2-infected fish. The results suggest that the combined vaccine exhibits a better protective effect compared to the single-antigen vaccines, indicating it as a promising approach to prevent CyHV-2 outbreaks.
鲤疱疹病毒2型（CyHV-2）是造血组织坏死性疱疹病毒病（HVHN）的病原体，给鲫鱼造成了巨大的经济损失。尽管已经研发出多种口服疫苗来预防CyHV-2，但它们并未取得理想的保护效果。为提高口服疫苗的保护效果，通过将在细胞表面展示ORF132或ORF25的重组酿酒酵母按1:1比例混合制成联合疫苗。用这种联合疫苗进行口服免疫可诱导鱼血清中产生针对ORF132和ORF25的特异性抗体，同时提高脾脏、头肾和后肠中IgT、IgM、IL-1β、IFN-1和TNF-α的转录水平。重要的是，联合疫苗为鲫鱼抵抗CyHV-2感染提供了显著保护，相对存活率达80.77%。此外，它还抑制了病毒载量，减轻了CyHV-2感染鱼的病理损伤。结果表明，与单抗原疫苗相比，联合疫苗具有更好的保护效果，表明它是预防CyHV-2爆发的一种有前景的方法。
