Berrios Louis, Ansell T Bertie, Dahlberg Peter D, Peay Kabir G
Department of Biology, Stanford University, Stanford, CA 94305, United States.
Division of CryoEM and Bioimaging, SSRL, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, United States.
FEMS Microbiol Rev. 2025 Jan 14;49. doi: 10.1093/femsre/fuae035.
Bacteria and ectomycorrhizal fungi (EcMF) represent two of the most dominant plant root-associated microbial groups on Earth, and their interactions continue to gain recognition as significant factors that shape forest health and resilience. Yet, we currently lack a focused review that explains the state of bacteria-EcMF interaction research in the context of experimental approaches and technological advancements. To these ends, we illustrate the utility of studying bacteria-EcMF interactions, detail outstanding questions, outline research priorities in the field, and provide a suite of approaches that can be used to promote experimental reproducibility, field advancement, and collaboration. Though this review centers on the ecology of bacteria, EcMF, and trees, it by default offers experimental and conceptual insights that can be adapted to various subfields of microbiology and microbial ecology.
细菌和外生菌根真菌（EcMF）是地球上最主要的两类与植物根系相关的微生物群体，它们之间的相互作用作为影响森林健康和恢复力的重要因素，日益受到关注。然而，目前我们缺乏一篇有针对性的综述，来阐述在实验方法和技术进步背景下细菌与外生菌根真菌相互作用的研究现状。为此，我们阐述了研究细菌与外生菌根真菌相互作用的实用性，详述了悬而未决的问题，概述了该领域的研究重点，并提供了一系列可用于提高实验可重复性、推动实地研究进展及促进合作的方法。尽管本综述聚焦于细菌、外生菌根真菌和树木的生态学，但默认提供了可应用于微生物学和微生物生态学各个子领域的实验和概念性见解。
