肺炎克雷伯菌多重耐药临床分离株的β-内酰胺酶基因鉴定及分子基因分型
Identification of beta-lactamase genes and molecular genotyping of multidrug-resistant clinical isolates of Klebsiella pneumoniae.
作者信息
Ferdosi-Shahandashti Azadeh, Pournajaf Abazar, Ferdosi-Shahandashti Elaheh, Zaboli Fatemeh, Javadi Kasra
机构信息
Department of Microbiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran.
Infectious Diseases and Tropical Medicine Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
出版信息
BMC Microbiol. 2024 Dec 28;24(1):549. doi: 10.1186/s12866-024-03679-6.
BACKGROUND
Klebsiella pneumoniae is a clinically relevant pathogen that has raised considerable public health concerns. This study aims to determine the presence of beta-lactamase genes and perform molecular genotyping of multidrug-resistant (MDR) K. pneumoniae clinical isolates.
METHODS
Clinical isolates of MDR K. pneumoniae were collected from educational hospitals affiliated with Babol University of Medical Sciences. The isolates of K. pneumoniae were identified through standard microbial and biochemical tests. Antibiotic resistance was assessed using disk diffusion, modified Hodge test (MHT), combined disk, and polymerase chain reaction (PCR) methods. Enterobacterial Repetitive Intergenic Consensus (ERIC)-PCR was performed for molecular typing.
RESULTS
A total of 42 MDR K. pneumoniae isolates were obtained from various clinical specimens. The highest antibiotic resistance was observed for ampicillin (100%), while the lowest resistance was noted for amikacin (19.04%). The MHT indicated that 38.09% of K. pneumoniae isolates produced carbapenemase enzymes. Metallo-beta-lactamase (MBL) production was found in 54.76% of isolates. Molecular detection of beta-lactamase genes revealed the presence of bla (21.42%), bla (42.85%), bla (76.19%), bla (47.16%), and bla (80.95%) genes. ERIC-PCR molecular typing identified seven distinct genetic patterns among the isolates.
CONCLUSIONS
This investigation demonstrates the high resistance levels of K. pneumoniae strains. The beta-lactamase genes with the highest and lowest frequencies correspond to bla and bla genes, respectively. ERIC-PCR dendrograms suggest a common origin for K. pneumoniae clinical isolates and the propagation of similar clones within hospital wards. These findings indicate that K. pneumoniae isolates are highly virulent, necessitating the development of more effective resistance-fighting techniques and gene transfer research.
CLINICAL TRIAL NUMBER
Not applicable.
背景
肺炎克雷伯菌是一种具有临床相关性的病原体，引起了相当大的公共卫生关注。本研究旨在确定β-内酰胺酶基因的存在，并对多重耐药（MDR）肺炎克雷伯菌临床分离株进行分子基因分型。
方法
从巴博尔医科大学附属教学医院收集MDR肺炎克雷伯菌的临床分离株。通过标准微生物学和生化试验鉴定肺炎克雷伯菌分离株。使用纸片扩散法、改良 Hodge 试验（MHT）、复合纸片法和聚合酶链反应（PCR）方法评估抗生素耐药性。采用肠杆菌重复基因间共识（ERIC）-PCR 进行分子分型。
结果
从各种临床标本中总共获得了42株MDR肺炎克雷伯菌分离株。氨苄西林的耐药率最高（100%），而阿米卡星的耐药率最低（19.04%）。MHT表明38.09%的肺炎克雷伯菌分离株产生碳青霉烯酶。54.76%的分离株检测到金属β-内酰胺酶（MBL）产生。β-内酰胺酶基因的分子检测显示存在bla（21.42%）、bla（42.85%）、bla（76.19%）、bla（47.16%）和bla（80.95%）基因。ERIC-PCR分子分型在分离株中鉴定出七种不同的遗传模式。
结论
本研究表明肺炎克雷伯菌菌株具有较高的耐药水平。频率最高和最低的β-内酰胺酶基因分别对应于bla和bla基因。ERIC-PCR树状图表明肺炎克雷伯菌临床分离株有共同的起源，且相似克隆在医院病房内传播。这些发现表明肺炎克雷伯菌分离株具有高度致病性，需要开发更有效的抗耐药技术和基因转移研究。
临床试验编号
不适用。
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