Li Yin, Song Guangyuan, Jiang Yu, Zhao Haitao, Zhu Yizhun, Song Shanshan, Wang Lulu, Wu Xueying
Department of Stomatology, Beijing Jishuitan Hospital, Capital Medical University, Beijing, 100035, China.
Beijing Engineering Research Center of Immunocellular Therapy, Beijing, China.
Sci Rep. 2024 Dec 28;14(1):31279. doi: 10.1038/s41598-024-82734-8.
Mesenchymal stem cells (MSCs) have been widely used in the treatment of various inflammatory diseases. The inadequate understanding of MSCs and their heterogeneity can impact the immune environment, which may be the cause of the good outcomes of MSCs-based therapy that cannot always be achieved. Recently, stem cells from human exfoliated deciduous teeth (SHED) showed great potential in inflammatory and autoimmune diseases due to their immature properties compared with MSCs. In our study, single-cell RNA sequencing (scRNA-seq) revealed that SHED in a low differentiation state (S7) exhibited the powerful ability to recruit multiple immune cells. In contrast, SHED in a relatively high differentiation state (S1) may hold a solid ability to secret many factors with paracrine signaling capacity. The analysis result shows that SHED has more robust immunomodulatory properties than human bone marrow-derived mesenchymal stem cells (hBMSCs) or human umbilical cord-derived mesenchymal stem cells (hUCMSCs). When co-cultured with PBMCs, SHED can enhance the proliferation of Treg and down-regulate TNF-α in vitro. SHED may have some advantages in the treatment of inflammatory and autoimmune diseases.
间充质干细胞（MSCs）已被广泛应用于各种炎症性疾病的治疗。对MSCs及其异质性的认识不足会影响免疫环境，这可能是基于MSCs的治疗并非总能取得良好效果的原因。最近，人脱落乳牙干细胞（SHED）由于其与MSCs相比具有不成熟的特性，在炎症和自身免疫性疾病中显示出巨大潜力。在我们的研究中，单细胞RNA测序（scRNA-seq）显示，低分化状态的SHED（S7）表现出强大的招募多种免疫细胞的能力。相比之下，相对高分化状态的SHED（S1）可能具有较强的分泌许多具有旁分泌信号能力的因子的能力。分析结果表明，SHED比人骨髓间充质干细胞（hBMSCs）或人脐带间充质干细胞（hUCMSCs）具有更强的免疫调节特性。与外周血单核细胞（PBMCs）共培养时，SHED在体外可增强调节性T细胞（Treg）的增殖并下调肿瘤坏死因子-α（TNF-α）。SHED在炎症和自身免疫性疾病的治疗中可能具有一些优势。
