Grantz Jillian M, Thirumalaikumar Venkatesh P, Jannasch Amber H, Andolino Chaylen, Taechachokevivat Natnicha, Avila-Granados Lisa M, Neves Rafael C
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Purdue University, West Lafayette, IN, 47907, USA.
Purdue Proteomics Facility, Bindley Bioscience Center, Purdue University, West Lafayette, IN, 47907, USA.
Sci Rep. 2024 Dec 28;14(1):31240. doi: 10.1038/s41598-024-82553-x.
Unregulated, systemic inflammation negatively impacts health and production in dairy cows. Soluble mediators and platelets have been studied for their expansive role in mediating inflammation. Our objectives were to compare the plasma oxylipin and endocannabinoid profiles, and the platelet and plasma proteomic profiles of healthy cows to cows experiencing elevated systemic inflammation as indicated by plasma haptoglobin (Hp) concentrations. Postpartum cows at 3 DIM with plasma Hp concentrations [Formula: see text] 0.50 g/L and no clinical disease were enrolled into the high-inflammation group (n = 8). Cows with plasma Hp concentrations [Formula: see text] 0.1 g/L and no clinical disease were enrolled into the low-inflammation group (n = 8). Targeted lipidomic analysis revealed differences in the plasma oxylipin and endocannabinoid profile between high- and low-inflammation cows. Cows in the high-inflammation group had increased plasma concentrations of the oxylipins 9(S)-HpOTrE, 9(S)-HOTrE, 13(S)-HpOTrE, and 9,10-EpOME, and the endocannabinoid anandamide. In-depth proteomic analysis of platelets between the high- and low-inflammation groups revealed significant differences in protein categories related to platelet granule release and cellular iron uptake. Proteomic outputs from plasma revealed 24 proteins to be different between high and low-inflammation groups, including proteins involved in autophagy and immune mediation. Together, our results indicate that cows experiencing an exacerbated systemic inflammatory response in the postpartum may have impaired disease resistance, and platelets could be contributors to their inflammatory state.
不受控制的全身性炎症会对奶牛的健康和生产产生负面影响。可溶性介质和血小板在介导炎症方面的广泛作用已得到研究。我们的目标是比较健康奶牛与血浆触珠蛋白（Hp）浓度表明全身性炎症升高的奶牛的血浆氧化脂质和内源性大麻素谱，以及血小板和血浆蛋白质组谱。将产后3天、血浆Hp浓度≥0.50 g/L且无临床疾病的奶牛纳入高炎症组（n = 8）。将血浆Hp浓度≤0.1 g/L且无临床疾病的奶牛纳入低炎症组（n = 8）。靶向脂质组学分析揭示了高炎症和低炎症奶牛之间血浆氧化脂质和内源性大麻素谱的差异。高炎症组奶牛的氧化脂质9(S)-HpOTrE、9(S)-HOTrE、13(S)-HpOTrE和9,10-EpOME以及内源性大麻素花生四烯乙醇胺的血浆浓度升高。高炎症组和低炎症组之间血小板的深入蛋白质组学分析揭示了与血小板颗粒释放和细胞铁摄取相关的蛋白质类别存在显著差异。血浆蛋白质组学结果显示，高炎症组和低炎症组之间有24种蛋白质不同，包括参与自噬和免疫介导的蛋白质。总之，我们的结果表明，产后全身性炎症反应加剧的奶牛可能抗病能力受损，血小板可能是其炎症状态的促成因素。
