Irdianto Shadira Anindieta, Fadhillah Fadhillah, Lestari Retno, Fadilah Fadilah, Bowolaksono Anom, Dwiranti Astari
Cellular and Molecular Mechanisms in Biological System (CEMBIOS) Research Group, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok, Indonesia.
Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia, Central Jakarta, Indonesia.
Microsc Res Tech. 2025 Apr;88(4):1239-1247. doi: 10.1002/jemt.24780. Epub 2024 Dec 29.
This study delves into the intriguing world of extrachromosomal DNA (ecDNA) in breast cancer, uncovering its pivotal role in cancer's aggressiveness and genetic variability. ecDNA, a form of circular DNA found outside chromosomes, is known to play a significant role in cancer progression by increasing oncogene expression. Focusing on two contrasting cell lines, MDA-MB-231 (triple-negative) and MCF-7 (Luminal-A), we utilized advanced microscopy and fluorescence techniques to detect and characterize ecDNA. Our findings reveal a stark difference: MDA-MB-231 cells, known for their high metastatic potential, exhibit a striking abundance of ecDNA, manifested as double minutes and single form with intense fluorescence signals. In contrast, the less aggressive MCF-7 cells harbor significantly fewer ecDNA. This disparity highlights the potential of ecDNA as a key player in cancer progression and a promising target for novel therapies. This research sheds light on the unseen genetic forces driving breast cancer and opens the door to new strategies in cancer treatment. Further research is necessary to understand the mechanisms of ecDNA formation and its role in different breast cancer subtypes.
本研究深入探讨了乳腺癌中染色体外DNA（ecDNA）这一引人入胜的领域，揭示了其在癌症侵袭性和基因变异性中所起的关键作用。ecDNA是一种存在于染色体外的环状DNA形式，已知通过增加癌基因表达在癌症进展中发挥重要作用。我们聚焦于两种截然不同的细胞系，MDA-MB-231（三阴性）和MCF-7（腔面A型），利用先进的显微镜和荧光技术来检测和表征ecDNA。我们的研究结果揭示了一个显著差异：以高转移潜能著称的MDA-MB-231细胞表现出大量的ecDNA，呈现为双微体和具有强烈荧光信号的单形式。相比之下，侵袭性较低的MCF-7细胞含有的ecDNA明显较少。这种差异凸显了ecDNA作为癌症进展关键因素以及新型治疗方法潜在靶点的可能性。这项研究揭示了驱动乳腺癌的无形遗传力量，为癌症治疗的新策略打开了大门。有必要进一步开展研究以了解ecDNA的形成机制及其在不同乳腺癌亚型中的作用。
