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Quantitative electron probe x-ray microanalysis and densitometric mass determination of individual rat blood platelets.

作者信息

Linders P W, Van de Vorstenbosch R A, Stadhouders A M

出版信息

J Histochem Cytochem. 1985 Mar;33(3):185-90. doi: 10.1177/33.3.3973377.

DOI:10.1177/33.3.3973377
PMID:3973377
Abstract

An improved method for quantitative electron probe X-ray microanalysis of thin biological specimens, introduced recently, has been applied to the elemental analysis of rat blood platelets. The method uses the X-ray signal to quantify the elemental content of an object and electron scattering to determine the total dry mass of the object. Along with the dry mass distribution, data were obtained on the content and mass fraction of calcium, magnesium, and sulfur in 31 individual platelets. The mean platelet dry mass was found to be 985 fg. The mean Ca, Mg, and S contents were 1.80, 3.41, and 18.3 fg, with mean dry mass fractions of 0.195, 0.396, and 1.96%, respectively. Furthermore, these elements appear to be unevenly distributed among the platelet population.

摘要

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引用本文的文献

1
Elemental analysis of individual rat blood platelets by electron probe X-ray microanalysis using a direct quantification method.采用直接定量法，通过电子探针X射线微量分析对单个大鼠血小板进行元素分析。
Histochemistry. 1985;82(3):257-61. doi: 10.1007/BF00501402.
2
The use of backscattered electrons in analytical electron microscopy for the measurement of the mass of individual rat blood platelets.在分析电子显微镜中使用背散射电子来测量单个大鼠血小板的质量。
Histochemistry. 1986;84(4-6):379-82. doi: 10.1007/BF00482966.