Stols A L, Stadhouders A M, Linders P W, van de Vorstenbosch R A
Histochemistry. 1986;84(4-6):379-82. doi: 10.1007/BF00482966.
The backscattered electron signal, generated in individual cells, has been used to measure the dry mass of these cells. Absolute mass values were obtained by comparing the backscattered electron signals of cells to the signals of polystyrene-latex spheres of known mass. The technique was carried out in an automated analytical scanning transmission electron microscope and applied to rat blood platelets. The resulting mass distributions agreed well with the distribution measured with a method that uses the transmitted electron signal by means of densitometric analysis of electrographs. Also the range of masses was in agreement with values deduced from data in the literature. The fully automated technique has the advantage that it is direct, fast, and that thicker specimens can be measured than is possible using the transmitted electron signal. The method is intended for use in combination with quantitative electron probe X-ray microanalysis and is then able to produce elemental mass fractions of biological specimens at the subcellular level.
在单个细胞中产生的背散射电子信号已被用于测量这些细胞的干质量。通过将细胞的背散射电子信号与已知质量的聚苯乙烯 - 乳胶球的信号进行比较，获得绝对质量值。该技术在自动分析扫描透射电子显微镜中进行，并应用于大鼠血小板。所得的质量分布与通过对电子图像进行密度分析使用透射电子信号测量的分布非常吻合。质量范围也与从文献数据推导的值一致。这种全自动技术的优点是直接、快速，并且与使用透射电子信号相比，可以测量更厚的标本。该方法旨在与定量电子探针X射线微分析结合使用，然后能够在亚细胞水平上产生生物标本的元素质量分数。
