Liang Suyun, Chang Zhuo, Lu Meixi, Guo Zhanbao, Luo Dawei, Xing Guangnan, Xie Ming, Huang Wei, Hou Shuisheng
Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Beijing General Station of Animal Husbandry, Beijing 100107, China.
Int J Biol Macromol. 2025 Mar;294:139168. doi: 10.1016/j.ijbiomac.2024.139168. Epub 2024 Dec 27.
Duck viral hepatitis (DVH) is a common and serious acute infectious disease that has a significantly impact on the duck farming industry. Duck hepatitis A virus type 3 (DHAV-3) is the major causative agent of DVH in East Asia. Host factor indicators of resistance to DHAV-3 in Pekin ducks were investigated using resistant (Z7R) and susceptible (Z7S) duck lines. Before DHAV-3 infection, Z7R had significantly higher HDL-C and LDL-C levels than Z7S. The results of population verification showed that Pekin ducks with HDL-C and/or LDL-C concentrations within their maximum 5 % confidence interval were highly resistant to DHAV-3. RNA-seq identified fifteen differentially expressed genes, primarily involved in lipid metabolism. Additionally, lipidomics identified one hundred distinct metabolites involved in glycerophospholipid metabolism. The ACSL6 gene was found to be significantly associated with PC and PE. ACSL6, PE, PC, HDL-C and LDL-C co-regulated hepatic lipid metabolism. In conclusion, our results reveal that HDL-C and LDL-C may serve as markers of anti-DHAV-3 infection and lipid metabolism may be related to a potential mechanism of antiviral activity in Pekin ducks, providing a theoretical basis for future studies on the interaction between lipid metabolism and DHAV-3.
鸭病毒性肝炎（DVH）是一种常见且严重的急性传染病，对养鸭业有重大影响。鸭甲型肝炎病毒3型（DHAV-3）是东亚地区DVH的主要致病因子。利用抗性（Z7R）和易感（Z7S）鸭系研究了北京鸭对DHAV-3抗性的宿主因子指标。在DHAV-3感染前，Z7R的高密度脂蛋白胆固醇（HDL-C）和低密度脂蛋白胆固醇（LDL-C）水平显著高于Z7S。群体验证结果表明，HDL-C和/或LDL-C浓度处于其最大5%置信区间内的北京鸭对DHAV-3具有高度抗性。RNA测序鉴定出15个差异表达基因，主要参与脂质代谢。此外，脂质组学鉴定出100种参与甘油磷脂代谢的不同代谢物。发现酰基辅酶A合成酶长链家族成员6（ACSL6）基因与磷脂酰胆碱（PC）和磷脂酰乙醇胺（PE）显著相关。ACSL6、PE、PC、HDL-C和LDL-C共同调节肝脏脂质代谢。总之，我们的结果表明，HDL-C和LDL-C可能作为抗DHAV-3感染的标志物，脂质代谢可能与北京鸭抗病毒活性的潜在机制有关，为未来脂质代谢与DHAV-3相互作用的研究提供了理论基础。
