Fan Xinxin, Lu Yu, Xie Yujun, Yan Xiaodong, Fan Wanyu, Ishii Yuji, Yu Boyang, Li Renshi
Jiangsu Provincial Key Laboratory of TCM Evaluation and Translational Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China; Laboratory of Molecular Life Sciences, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Laboratory of Molecular Life Sciences, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Int J Biol Macromol. 2025 Mar;293:139216. doi: 10.1016/j.ijbiomac.2024.139216. Epub 2024 Dec 27.
Anemarrhena asphodeloides Bge. (AAB), a traditional medicinal herb, has a long history of delaying the aging process. Yet, the anti-aging effects of its polysaccharides have not been thoroughly investigated. This study marks the first exploration of the anti-aging activity of Anemarrhena asphodeloides Bge. polysaccharides (AABP). The MW of AABP-1a was determined to be 210.062 kDa, with a composition consisting predominantly of glucose and mannose in a molar ratio of approximately 4:1. The backbone of AABP-1a was mainly composed of →4)-2Ac-β-Man(1→ and →4)-β-Glc(1→ and a small amount of branched →4,6)-β-Glc(1→ and →3,4)-β-Glc(1→, the branching part was composed of →6)-β-Glc(1→ and t-α-Glc(1→. AABP-1a has antioxidant capacity and can improve cell cycle arrest mediated by senescence markers such as p53, p21, p16 and SASP, and reduce the accumulation of damaged DNA. In addition, it could reduce the activity of SA-β-Gal in zebrafish, prolong the lifespan of C. elegans and reduce the expression of lipofuscin. This study found a glucomannan and demonstrated its anti-aging activity in various aging models. These results provide a theoretical basis for further study of the anti-aging effect of AAB.
知母（Anemarrhena asphodeloides Bge.，AAB）是一种传统草药，在延缓衰老过程方面有着悠久的历史。然而，其多糖的抗衰老作用尚未得到充分研究。本研究首次探索了知母多糖（AABP）的抗衰老活性。AABP-1a的分子量测定为210.062 kDa，其组成主要为葡萄糖和甘露糖，摩尔比约为4:1。AABP-1a的主链主要由→4)-2Ac-β-Man(1→和→4)-β-Glc(1→组成，还有少量分支的→4,6)-β-Glc(1→和→3,4)-β-Glc(1→，分支部分由→6)-β-Glc(1→和t-α-Glc(1→组成。AABP-1a具有抗氧化能力，可改善由衰老标志物如p53、p21、p16和SASP介导的细胞周期阻滞，并减少受损DNA的积累。此外，它可以降低斑马鱼中SA-β-Gal的活性，延长秀丽隐杆线虫的寿命并减少脂褐素的表达。本研究发现了一种葡甘露聚糖，并在多种衰老模型中证明了其抗衰老活性。这些结果为进一步研究知母的抗衰老作用提供了理论依据。
