LeCrone C N, Jones J A, Detter J C
Am J Med Technol. 1983 Mar;49(3):165-7.
We describe a subject who had a positive sickle solubility test and a routine alkaline electrophoretic pattern showing three major hemoglobulin bands migrating to the A, S, and A2 positions. In addition, the non-heme protein carbonic anhydrase appeared to be increased, suggesting the presence of a split A2. Two major hemoglobin bands separated on agar gel electrophoresis using a citrate buffer, pH 6.2. A non-S hemoglobin migrating to the position of Hb S was identified as GPhiladelphia. The electrophoretic pattern of GPhil/S trait most closely resembles S/beta+ thalassemia or sickle cell trait. Careful study is essential to avoid misdiagnosis and unfairly stigmatizing patients whose benign abnormality may be confused with more severe disease entities.
我们描述了一名受试者，其镰状细胞溶解度试验呈阳性，常规碱性电泳图谱显示有三条主要血红蛋白带迁移至A、S和A2位置。此外，非血红素蛋白碳酸酐酶似乎有所增加，提示存在分裂型A2。使用pH 6.2的柠檬酸盐缓冲液在琼脂凝胶电泳上分离出两条主要血红蛋白带。一条迁移至Hb S位置的非S血红蛋白被鉴定为G费城。G费城/ S性状的电泳图谱与S/β+地中海贫血或镰状细胞性状最为相似。仔细研究对于避免误诊以及不公平地给那些良性异常可能与更严重疾病实体相混淆的患者贴上标签至关重要。
