Liu Jinrong, Qi Jin, Li Jiadi, Zhang Tong, Ren Jianing, Zhang Zheyuan, Ning Xiao, Zhang Ran, Liu Xiaoming, Li Bing, Wu Xiuping
Shanxi Medical University School and Hospital of Stomatology; Shanxi Province Key Laboratory of Oral Diseases Prevention and New Materials, Taiyuan, Shanxi, 030001, People's Republic of China.
Int J Nanomedicine. 2024 Dec 23;19:13823-13838. doi: 10.2147/IJN.S495706. eCollection 2024.
During fixed orthodontic treatment, oral hygiene is difficult to ensure and can easily lead to an imbalance in the oral micro-ecological balance. In this study, based on the adhesive properties of polydopamine (PDA) and the good antimicrobial and remineralization properties of carboxymethyl chitosan (CMC) and xylitol (Xy), new nanocomposites with both antimicrobial and remineralization capabilities were prepared to coat on orthodontic brackets.
Composite carbon dots (CDs) were synthesized using carboxymethyl chitosan and xylitol, we characterized them and the antimicrobial properties of the CMC-Xy-CDs were investigated by co-cultivation with S. mutans in vitro and in vivo. The composite coating was then adhered to the brackets. After the characterization measurements, antibacterial properties against S. mutans and the ability to promote remineralization of isolated teeth were investigated.
Biological studies demonstrated that L929 cells co-cultured with CMC-Xy-CDs did not observe significant cytotoxicity and CMC-Xy-CDs have good biocompatibility. In the adhesive action of PDA, various characterizations have proved that CDs are modified on the brackets surface. In the antibacterial experiment, CMC-Xy-CDs and the adhesive coating on the brackets were found to have better antibacterial performance than the control group, with an antibacterial rate of up to 80%. In the animal experiment, the results of CMC-Xy-CDs promoting the healing of S. mutans infection wound models showed that there was a significant difference at 7d (P < 0.001), indicating that the experimental group had good antibacterial effects. The SEM of teeth after CMC-Xy-CDs promoted remineralization showed that the structure of the tooth surface became dense and some precipitation appeared, and the surface hardness measurement was significantly increased (p<0.0001).
Our study revealed that new nanocomposites with both antimicrobial and remineralization capabilities coated on orthodontic brackets provide a good basis for future clinical applications.
在固定正畸治疗期间，口腔卫生难以保证，容易导致口腔微生态平衡失调。本研究基于聚多巴胺（PDA）的黏附特性以及羧甲基壳聚糖（CMC）和木糖醇（Xy）良好的抗菌及再矿化特性，制备具有抗菌和再矿化双重功能的新型纳米复合材料，用于包被正畸托槽。
采用羧甲基壳聚糖和木糖醇合成复合碳点（CDs），对其进行表征，并通过与变形链球菌体外和体内共培养研究CMC-Xy-CDs的抗菌性能。然后将复合涂层黏附于托槽上。在进行表征测量后，研究其对变形链球菌的抗菌性能以及对离体牙再矿化的促进能力。
生物学研究表明，与CMC-Xy-CDs共培养的L929细胞未观察到明显的细胞毒性，CMC-Xy-CDs具有良好的生物相容性。在PDA的黏附作用下，各种表征证明CDs修饰在了托槽表面。在抗菌实验中，发现CMC-Xy-CDs及托槽上的黏附涂层比对照组具有更好的抗菌性能，抗菌率高达80%。在动物实验中，CMC-Xy-CDs促进变形链球菌感染伤口模型愈合的结果显示，在第7天时存在显著差异（P<0.001），表明实验组具有良好的抗菌效果。CMC-Xy-CDs促进再矿化后牙齿的扫描电镜结果显示，牙齿表面结构变得致密，出现了一些沉淀，表面硬度测量值显著增加（p<0.0001）。
我们的研究表明，包被在正畸托槽上的具有抗菌和再矿化双重功能的新型纳米复合材料为未来的临床应用提供了良好的基础。
