Allahmoradi Mahya, Najafi Faezeh, Koosha Mona, Kazemirad Elham, Latifi Alireza, Naddaf Saied Reza, Mirjalali Hamed, Mohebali Mehdi, Hajjaran Homa, Otranto Domenico, Mowlavi Gholamreza
Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Department of Vector Biology and Control of Diseases, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Iran J Parasitol. 2024 Oct-Dec;19(4):496-501. doi: 10.18502/ijpa.v19i4.17172.
Rodents are the primary reservoir hosts for zoonotic cutaneous leishmaniasis (ZCL) caused by . Knowing reservoir hosts is crucial for leishmaniasis surveillance and control programs in endemic areas. In this study, we examined an archived spleen of obtained during a pest control program in 2000 in Tehran, the capital of Iran. The sample was analyzed using polymerase chain reaction (PCR) and sequencing to determine the presence of Trypanosomatidae based on the internal transcribed spacer () 1 gene. Amplification and sequencing of the discriminative region of the gene followed by BLAST analysis showed the highest similarity with isolates. Also, the phylogenetic analysis revealed that our sample was grouped with isolates retrieved from the GenBank database. This finding might support the claim that acts as a potential reservoir host for . Further studies, including a survey on more rodent samples as well as studying sandflies in the area, might uncover the possible presence of such pathobiological conditions in ZCL transmission in urban and suburban settings.
啮齿动物是由……引起的人兽共患皮肤利什曼病（ZCL）的主要储存宿主。了解储存宿主对于流行地区的利什曼病监测和控制计划至关重要。在本研究中，我们检测了一份于2000年在伊朗首都德黑兰的一次害虫防治计划中获取的存档脾脏。使用聚合酶链反应（PCR）和测序对样本进行分析，以基于内转录间隔区（ITS）1基因确定锥虫科的存在。对ITS基因的鉴别区域进行扩增和测序，随后进行BLAST分析，结果显示与……分离株的相似度最高。此外，系统发育分析表明，我们的样本与从GenBank数据库中检索到的……分离株归为一类。这一发现可能支持……作为……潜在储存宿主的说法。进一步的研究，包括对更多啮齿动物样本的调查以及对该地区白蛉的研究，可能会揭示城市和郊区环境中ZCL传播中此类病理生物学状况的可能存在。
