Azimi Taher, Pourmand Mohammad Reza, Fallah Fatemeh, Karimi Abdollah, Mansour-Ghanaie Roxana, Hoseini-Alfatemi Seyedeh Mahsan, Shirdoust Mehdi, Azimi Leila
Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Students Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Trop Med Health. 2020 Jul 22;48:60. doi: 10.1186/s41182-020-00246-3. eCollection 2020.
are reservoirs for transmission of various zoonotic parasites, and they have become a threat to public health worldwide. Given the large number and the significant presence of throughout the city of Tehran, this study aims to assess the frequency of zoonotic parasites carried by commensal rodents wandering in Tehran, Iran. The study considered the north, south, west, east, and center regions of Tehran for the purposes of this study. The serological tests were applied in order to detect effective antibodies against (), spp., and spp. using a commercial qualitative rat ELISA kit. The frequency of () was surveyed by using the conventional PCR method. Furthermore, nested PCR was employed to detect the presence of spp. and spp. in commensal dispersed in Tehran.
Approximately, 76% of the 100 tested were infected with at least one zoonotic parasite, indicating the significant frequency of parasites within the study areas. Seroreactivity against , spp., and spp. was detected in 5%, 0%, and 1% of the tested, respectively. DNA was detected in 32 out of 100 (32%) . In addition, spp. and spp. DNA were found in 18 out of 100 (18%) and 76 out of 100 (76%) investigated, respectively. with 15% and with 70% had the highest frequency of parasites among the collected from the western and northeastern regions of Tehran, respectively. Moreover, spp. with 95% and spp. with 30% had the highest frequency in the east and center districts, respectively.
The findings showed a wide geographical dissemination of spp., , and spp. in within five districts of Tehran. In contrast, other parasites such as spp. infection were rarely detected in populations. No evidence for the circulation of spp. was found in this study.
是多种人畜共患寄生虫的传播宿主，已成为全球公共卫生的一大威胁。鉴于德黑兰市大量存在且分布广泛，本研究旨在评估伊朗德黑兰四处游荡的共生啮齿动物所携带的人畜共患寄生虫的感染率。本研究将德黑兰的北部、南部、西部、东部和中部地区纳入研究范围。为检测针对（）、 spp. 和 spp. 的有效抗体，应用了血清学检测，使用的是商用定性大鼠ELISA试剂盒。采用常规PCR方法调查（）的感染率。此外，采用巢式PCR检测德黑兰各处共生体内spp. 和 spp. 的存在情况。
在检测的100只中，约76%感染了至少一种人畜共患寄生虫，表明研究区域内寄生虫感染率很高。在检测的中，分别有5%、0%和1%对、 spp. 和 spp. 呈血清反应阳性。在100只（32%）中检测到DNA。此外，在100只被调查的中，分别有18只（18%）和76只（76%）检测到spp. 和 spp. 的DNA。在从德黑兰西部地区收集的中，感染率最高，为15%；在从德黑兰东北部地区收集的中，感染率最高，为70%。此外，在东部地区，spp. 感染率最高，为95%；在中部地区，spp. 感染率最高，为30%。
研究结果表明，在德黑兰五个区的体内，spp.、和 spp. 广泛分布于地理区域。相比之下，在种群中很少检测到其他寄生虫感染，如spp. 感染。本研究未发现spp. 传播的证据。
