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实验室条件下及医院环境中内共生菌阳性样本的分子诊断

Molecular Diagnosis of Endosymbiont in -Positive Samples in Laboratory Conditions and in the Hospital Environments.

作者信息

Mohammadi Alireza, Dalimi Abdolhossein, Ghafarifar Fatemeh, Pirestani Majid, Akbari Majid

机构信息

Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

Department of Microbiology, Arak University of Medical Sciences, Arak, Iran.

出版信息

Iran J Parasitol. 2024 Oct-Dec;19(4):397-407. doi: 10.18502/ijpa.v19i4.17160.

DOI:10.18502/ijpa.v19i4.17160
PMID:39735838
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11671829/
Abstract

BACKGROUND

We aimed to identity endosymbiont in -positive samples in natural and laboratory conditions.

METHODS

Overall, 134 samples were collected from hospital environments. Microscopic and PCR test were used for detection of and The real-time PCR method was used to check the active presence of within under natural conditions from hospital samples and in co-culture laboratory conditions.

RESULTS

The rate of contamination of hospital samples with was 44.7%. Out of 42 PCR-positive samples, 13 isolates (31%) were positive in terms of endosymbiont according to sampling location. is able to penetrate and enter the parasite.

CONCLUSION

is able to contaminate in natural and laboratory conditions. The presence of pathogenic in various hospital environments and the hiding of as an endosymbiont inside it can pose a serious threat to the health of hospitalized patients.

摘要

背景

我们旨在鉴定自然和实验室条件下阳性样本中的内共生体。

方法

总共从医院环境中收集了134份样本。使用显微镜检查和PCR检测 和 。采用实时PCR方法检测医院样本在自然条件下以及在共培养实验室条件下 内 的活性存在情况。

结果

医院样本中 的污染率为44.7%。在42份PCR阳性样本中,根据采样位置,13株分离株(31%)的内共生体检测呈阳性。 能够穿透并进入 寄生虫。

结论

在自然和实验室条件下均能污染 。各种医院环境中致病性 的存在以及其作为内共生体隐藏在其中,可能对住院患者的健康构成严重威胁。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/451a/11671829/4eeb4eb04f9c/IJPA-19-397-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/451a/11671829/49e13f3a90b4/IJPA-19-397-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/451a/11671829/53adde9ea8c3/IJPA-19-397-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/451a/11671829/4f6b32f46dce/IJPA-19-397-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/451a/11671829/4eeb4eb04f9c/IJPA-19-397-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/451a/11671829/49e13f3a90b4/IJPA-19-397-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/451a/11671829/53adde9ea8c3/IJPA-19-397-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/451a/11671829/4f6b32f46dce/IJPA-19-397-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/451a/11671829/4eeb4eb04f9c/IJPA-19-397-g004.jpg

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Iran J Parasitol. 2023 Jan-Mar;18(1):38-47. doi: 10.18502/ijpa.v18i1.12378.
2
A Systematic Review of Intracellular Microorganisms within to Understand Potential Impact for Infection.关于细胞内微生物的系统评价,以了解其对感染的潜在影响。
Pathogens. 2021 Feb 18;10(2):225. doi: 10.3390/pathogens10020225.
3
Acanthamoeba castellanii supports extracellular survival of Helicobacter pylori in co-culture.
棘阿米巴原虫支持共培养中幽门螺杆菌的细胞外存活。
J Infect Chemother. 2020 Sep;26(9):946-954. doi: 10.1016/j.jiac.2020.04.016. Epub 2020 May 21.
4
Contamination of swimming pools and hot tubs biofilms with Acanthamoeba.棘阿米巴对游泳池和热水浴缸生物膜的污染。
Acta Parasitol. 2018 Mar 26;63(1):147-153. doi: 10.1515/ap-2018-0016.
5
Detection of Helicobacter pylori in stool samples of young children using real-time polymerase chain reaction.实时聚合酶链反应检测婴幼儿粪便样本中的幽门螺杆菌。
Helicobacter. 2018 Feb;23(1). doi: 10.1111/hel.12450. Epub 2017 Nov 27.
6
Genotyping of Acanthamoeba spp. from water sources from Northwestern Iran.对伊朗西北部水源中棘阿米巴属物种的基因分型。
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7
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9
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New Microbes New Infect. 2016 Nov 14;15:107-116. doi: 10.1016/j.nmni.2016.10.003. eCollection 2017 Jan.
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