实验室条件下及医院环境中内共生菌阳性样本的分子诊断
Molecular Diagnosis of Endosymbiont in -Positive Samples in Laboratory Conditions and in the Hospital Environments.
作者信息
Mohammadi Alireza, Dalimi Abdolhossein, Ghafarifar Fatemeh, Pirestani Majid, Akbari Majid
机构信息
Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Department of Microbiology, Arak University of Medical Sciences, Arak, Iran.
出版信息
Iran J Parasitol. 2024 Oct-Dec;19(4):397-407. doi: 10.18502/ijpa.v19i4.17160.
BACKGROUND
We aimed to identity endosymbiont in -positive samples in natural and laboratory conditions.
METHODS
Overall, 134 samples were collected from hospital environments. Microscopic and PCR test were used for detection of and The real-time PCR method was used to check the active presence of within under natural conditions from hospital samples and in co-culture laboratory conditions.
RESULTS
The rate of contamination of hospital samples with was 44.7%. Out of 42 PCR-positive samples, 13 isolates (31%) were positive in terms of endosymbiont according to sampling location. is able to penetrate and enter the parasite.
CONCLUSION
is able to contaminate in natural and laboratory conditions. The presence of pathogenic in various hospital environments and the hiding of as an endosymbiont inside it can pose a serious threat to the health of hospitalized patients.
背景
我们旨在鉴定自然和实验室条件下阳性样本中的内共生体。
方法
总共从医院环境中收集了134份样本。使用显微镜检查和PCR检测 和 。采用实时PCR方法检测医院样本在自然条件下以及在共培养实验室条件下 内 的活性存在情况。
结果
医院样本中 的污染率为44.7%。在42份PCR阳性样本中，根据采样位置，13株分离株（31%）的内共生体检测呈阳性。 能够穿透并进入 寄生虫。
结论
在自然和实验室条件下均能污染 。各种医院环境中致病性 的存在以及其作为内共生体隐藏在其中，可能对住院患者的健康构成严重威胁。
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