Institute of Laboratory Animals, Graduate School of Medicine, Kyorin University, 6-20-2 Shinkawa, Mitaka, Tokyo, Japan.
Department of Infectious Diseases, Kyorin University School of Medicine, 6-20-2 Shinkawa, Mitaka, Tokyo, Japan.
J Infect Chemother. 2020 Sep;26(9):946-954. doi: 10.1016/j.jiac.2020.04.016. Epub 2020 May 21.
This study aimed to demonstrate whether Helicobacter pylori is able to survive in co-culture with a protozoan, Acanthamoeba castellanii, in order to further investigate a possible aqueous environmental mode of transmission. Numbers of H. pylori in co-culture with A castellanii were assessed by colony forming unit (CFU) assay and cell morphology was observed by electron microscopy. Viable and intact H. pylori in co-culture were detected and the number of H. pylori in co-culture with A. castellanii was significantly higher than in bacterial single culture. It was also shown that co-culture of H. pylori with A. castellanii physically separated by a filter membrane negated this survival effect, suggesting that adherence of H. pylori to A. castellanii affects its survival. Scanning electron microscopy revealed helical forms of H. pylori in co-culture with A. castellanii, but not in single culture. These results imply that mutual interaction between H. pylori and A. castellanii in the environment is critical for survival of H. pylori. In addition, the H. pylori gene expression profile was found to differ between single and co-cultured cells using RNA-sequence analysis.
本研究旨在探讨幽门螺杆菌（Helicobacter pylori）是否能在与原生动物棘阿米巴（Acanthamoeba castellanii）的共培养中存活，以进一步研究其可能的水生环境传播方式。通过集落形成单位（CFU）测定评估共培养中幽门螺杆菌的数量，并通过电子显微镜观察细胞形态。检测共培养中存活的完整幽门螺杆菌，并发现共培养中幽门螺杆菌的数量明显高于细菌单独培养。此外，通过滤膜将幽门螺杆菌与棘阿米巴物理分离的共培养实验否定了这种生存效应，表明幽门螺杆菌对棘阿米巴的黏附影响其存活。扫描电子显微镜显示共培养中的幽门螺杆菌呈螺旋状，但在单独培养中则不是。这些结果表明，幽门螺杆菌和棘阿米巴在环境中的相互作用对于幽门螺杆菌的存活至关重要。此外，使用 RNA 测序分析发现，幽门螺杆菌在单培养和共培养细胞中的基因表达谱不同。
