Mohammadi Alireza, Dalimi Abdolhossein, Ghaffarifar Fatemeh, Pirestani Majid, Akbari Majid
Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Department of Microbiology, Arak University of Medical Sciences, Arak, Iran.
J Parasitol Res. 2025 Feb 14;2025:6626888. doi: 10.1155/japr/6626888. eCollection 2025.
Most s contain endosymbionts such as viruses, yeasts, protists, and bacteria, some of which are potential human pathogens, including which often causes gastroenteritis and septicemia in humans. Amoebae have been shown to be resistant to chlorination and apparently protect ingested bacteria such as from free chlorine. Such resistance can have health implications, especially for drinking water treatment. The aim of this study is to identify in hospital samples in Markazi province, to determine the identity of endosymbiont in positive samples of in natural and laboratory conditions, and to determine the relationship between the two. The main aim of this study was to determine the identity of endosymbiont in -positive samples in natural and laboratory conditions. In this study, 134 samples including water, soil, and dust were collected from hospital environments. After molecular detection, the identity of the symbiotic in was determined by microscopic and PCR methods. Then, the ability of bacteria to infect the parasite was examined by cocultivation in vitro using real-time PCR. Finally, their relationship was examined based on statistical tests. The rate of contamination of hospital samples with was 44.7% on average. Out of 42 PCR-positive samples, seven isolates (16.67%) were found to be positive in terms of endosymbiont according to sampling location. The results showed that is able to penetrate and enter the parasite. In conclusion, our results showed that is able to contaminate in natural and laboratory conditions. The presence of pathogenic in various hospital environments and the hiding of as an endosymbiont inside it can pose a serious threat to the health of hospitalized patients.
大多数变形虫含有内共生体，如病毒、酵母、原生生物和细菌，其中一些是潜在的人类病原体，包括通常会导致人类肠胃炎和败血症的[具体细菌名称未给出]。变形虫已被证明对氯化有抗性，并且显然能保护摄入的细菌，如[具体细菌名称未给出]免受游离氯的影响。这种抗性可能对健康有影响，尤其是对饮用水处理。本研究的目的是在马尔卡齐省的医院样本中鉴定[具体研究对象未明确给出]，确定自然和实验室条件下[具体研究对象未明确给出]阳性样本中内共生体的身份，并确定两者之间的关系。本研究的主要目的是确定自然和实验室条件下[具体研究对象未明确给出]阳性样本中内共生体的身份。在本研究中，从医院环境中收集了134份包括水、土壤和灰尘的样本。经过分子检测后，通过显微镜和PCR方法确定了[具体研究对象未明确给出]中共生[具体研究对象未明确给出]的身份。然后，使用实时PCR通过体外共培养检查细菌感染寄生虫的能力。最后，基于统计测试检查它们之间的关系。医院样本中[具体研究对象未明确给出]的污染率平均为44.7%。在42份[具体研究对象未明确给出]PCR阳性样本中，根据采样地点，有7株分离株（16.67%）在内共生体方面呈阳性。结果表明[具体细菌名称未给出]能够穿透并进入[具体研究对象未明确给出]寄生虫。总之，我们的结果表明[具体细菌名称未给出]能够在自然和实验室条件下污染[具体研究对象未明确给出]。各种医院环境中致病性[具体细菌名称未给出]的存在以及其作为内共生体隐藏在其中，可能对住院患者的健康构成严重威胁。
