Baqer Safa Hussein, Al-Younis Zena Kadhim, Al-Shawi Sarmad Ghazi
Food Science Department, Agriculture College, Basrah University, 61001 Basrah, Iraq.
Front Biosci (Elite Ed). 2024 Nov 25;16(4):35. doi: 10.31083/j.fbe1604035.
Flavonoids are among the most important compounds found in plants, since laboratory studies have shown them to be a daily requirement in human diets due to their various health benefits. Therefore, this study focused on extracting, purifying, and measuring the antioxidant activity of the flavonoid quercetin, which is widely found in plants and possesses a variety of biological activities, from different plant sources.
The extraction of quercetin was performed using several methods (chemical, physical, and enzymatic) and several extraction solutions (water, ethanol, and chloroform) from several plants (spinach, dill, Onion Skin, , sumac, digalkhasab chemri, and leelwi chemri). The alcoholic extract extracted by chemical method was purified and the content of total flavonoids based on quercetin in all plant extracts was determined using adsorption chromatography on a silica gel column (100-200 mesh), followed by thin layer chromatography (TLC). TLC and high performance liquid chromatography (HPLC) were used to assess the purity of quercetin. The ability of quercetin to capture free radicals using 2,2-diphenyl-1-picrylhydrazyl (DPPH) was compared to that of butylated hydroxytoluene (BHT). Statistical analyses were performed using completely randomized designs (CRD) for factorial experiments, and the least significant difference (LSD) test was used to calculate the significant differences between the averages of the coefficients at the 0.05 probability level.
The alcoholic extract extracted by chemical method yielded the highest concentration of quercetin (84.037 mg/g). Furthermore, it was found that quercetin purified from possessed strong antioxidant activity, and its antioxidant activity increased with increased concentration.
showed the highest quercetin content among the assessed plants. Moreover, solvents played a major role in extracting plant components due to the high polarity of flavonoids. Quercetin purified using a silica gel column demonstrated antioxidant activity.
黄酮类化合物是植物中发现的最重要的化合物之一，因为实验室研究表明，由于其具有多种健康益处，它们是人类日常饮食中的必需成分。因此，本研究聚焦于从不同植物来源中提取、纯化并测定黄酮类化合物槲皮素的抗氧化活性，槲皮素广泛存在于植物中并具有多种生物活性。
使用多种方法（化学法、物理法和酶法）以及多种提取溶剂（水、乙醇和氯仿）从多种植物（菠菜、莳萝、洋葱皮、漆树、地嘎尔哈萨布奇姆里和勒尔维奇姆里）中提取槲皮素。对化学法提取的醇提取物进行纯化，并使用硅胶柱（100 - 200目）上的吸附色谱法，随后进行薄层色谱法（TLC）测定所有植物提取物中基于槲皮素的总黄酮含量。使用TLC和高效液相色谱法（HPLC）评估槲皮素的纯度。将槲皮素使用2,2 - 二苯基 - 1 - 苦基肼（DPPH）捕获自由基的能力与丁基化羟基甲苯（BHT）的能力进行比较。使用完全随机设计（CRD）进行析因实验的统计分析，并使用最小显著差数法（LSD）检验计算在概率水平为0.05时系数平均值之间的显著差异。
化学法提取的醇提取物产生了最高浓度的槲皮素（84.037毫克/克）。此外，发现从[具体植物]纯化的槲皮素具有很强的抗氧化活性，并且其抗氧化活性随浓度增加而增强。
[具体植物]在所评估的植物中显示出最高的槲皮素含量。此外，由于黄酮类化合物的高极性，溶剂在提取植物成分中起主要作用。使用硅胶柱纯化的槲皮素表现出抗氧化活性。
