Mochamad Lazuardi, Chien Chi-Hsien, He Jie-Long, Hestianah Eka Pramyrtha, Plumeriastuti Hani
Sub-Division Veterinary Pharmacy Science, Faculty of Veterinary Medicine, Airlangga University, Mulyorejo Rd, "C" Campus Fakultas Kedokteran Hewan Universitas Airlangga, 3rd Floor Building, Surabaya 60115, Indonesia.
Department of Post-Baccalaureate Veterinary Medicine, Asia University, Taichung 41354, Taiwan.
Vet Med Int. 2025 May 25;2025:4081052. doi: 10.1155/vmi/4081052. eCollection 2025.
Quercetin-like compounds (QLCs) are secondary metabolite compounds of flavonol found in the leaf extract from the mistletoe L. Miq. This study aims to determine the ability of QLC to stimulate epithelialization in rabbit skin. The leaves were macerated with methanol, ethyl acetate, and n-hexane solvents, and crude macerates were separated and purified into QLC using preparative high-performance liquid chromatography. The purified QLC as an analyte was prepared in serial concentrations of 4.5%, 6%, 8%, and 10% and then formulated by new methods as topical gel preparations. The epithelialization stimulation test was performed on 60 rabbits divided into 20 rabbits for the trial group, the other 20 rabbits for the positive control group, and negative control groups. The trial group was split into 4 trial subgroups for topical gel application at each concentration. Gels were topically applied to the exfoliated skin of rabbits in the trial group twice a day for 5 days. The probit analysis showed that an epithelialization of 25%, 50%, and 75% of the in vivo study endpoint was found at a QLC concentration of 4.644%, 5.185%, and 5.790%. Rectangular cuboidal QLC particles with an average size of 0.01 μm-0.1 μm have shown the ability to stimulate epidermal epithelial proliferation starting from a concentration of 4.5% in topical gels with new formulations that can boost the epithelialization of the upper layers of the skin ( < 0.05).
槲皮素样化合物（QLCs）是从桑寄生L. Miq. 的叶提取物中发现的黄酮醇次生代谢产物。本研究旨在确定QLC刺激兔皮肤上皮形成的能力。将叶片用甲醇、乙酸乙酯和正己烷溶剂浸渍，粗浸膏经制备型高效液相色谱分离纯化得到QLC。将纯化后的QLC作为分析物，配制成4.5%、6%、8%和10%的系列浓度，然后采用新方法制成局部用凝胶制剂。对60只兔子进行上皮形成刺激试验，分为试验组20只兔子、阳性对照组20只兔子和阴性对照组。试验组分为4个试验亚组，分别应用每种浓度的局部用凝胶。试验组每天两次将凝胶局部涂抹于兔子的剥脱皮肤上，持续5天。概率分析表明，在QLC浓度为4.644%、5.185%和5.790%时，体内研究终点的上皮形成率分别为25%、50%和75%。平均尺寸为0.01μm - 0.1μm的长方体QLC颗粒已显示出能够从局部凝胶中4.5%的浓度开始刺激表皮上皮细胞增殖，这种新配方可以促进皮肤上层的上皮形成（<0.05）。
