Physical Chemistry of Natural Substances and Process Team, Laboratory of Applied Chemistry and Environment, Faculty of Sciences, University Mohamed Premier, Oujda, Morocco.
Laboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences, University Mohamed Premier, Oujda, Morocco.
ScientificWorldJournal. 2024 Feb 7;2024:1998870. doi: 10.1155/2024/1998870. eCollection 2024.
The aims of this study were to determine the polyphenolic profile, to estimate the total phenolic and flavonoid contents, and to evaluate the antioxidant and antidiabetic activities of the extract of leaves, and the hydroacetonic mixture was employed as an alternative for common solvents in the extraction process. In order to explain the antidiabetic activity, molecular docking has been performed on the main constituents of the leaf extract. The characterization of the extract has been performed by high-performance liquid chromatography (HPLC) leading to the detection of 20 compounds of which gallic acid, ellagic acid, catechin, kaempferol, and quercetin 3-glucoside were identified using authentic standards. The total phenolic and flavonoid contents, assessed using the Folin-Ciocalteu and quercetin methods, were 394.5 ± 0.08 mg gallic acid equivalent/g dry extract (mg GAE/g DE) and 101.2 ± 0.095 mg quercetin equivalent/g dry extract (mg QE/g DE), respectively. On the other hand, the antioxidant activity of leaf extract, quantified by determining the ability to neutralize the free radical DPPH and -carotene/linoleate model system, reached the values of 0.0027 ± 0.002 mg/mL and 0.128 ± 0.04 mg/mL, respectively. Regarding the antidiabetic activity, based on the inhibition of pancreatic -amylase activity, a significant inhibition of about 68.20% with an IC value of 0.266 mg/mL had been observed. This finding is consistent with the molecular docking study of the main phenolic compounds of the extracts, where a remarkable binding affinity against -amylase was observed, with values of -7.631 (kcal/mol), -6.818 (kcal/mol), and -5.517 (kcal/mol) for the major compounds catechin, quercetin-3-glucoside, and gallic acid, respectively.
本研究的目的是确定多酚的组成，估算总酚和类黄酮的含量，并评价叶提取物的抗氧化和抗糖尿病活性，水-乙酸乙酯混合物被用作提取过程中替代常用溶剂的方法。为了解释抗糖尿病活性，对叶提取物的主要成分进行了分子对接。采用高效液相色谱（HPLC）对提取物进行了表征，检测到 20 种化合物，其中使用标准品鉴定出了没食子酸、鞣花酸、儿茶素、山奈酚和槲皮素 3-葡萄糖苷。采用福林-肖卡（Folin-Ciocalteu）和槲皮素法测定的总酚和类黄酮含量分别为 394.5 ± 0.08 mg 没食子酸当量/克干提取物（mg GAE/g DE）和 101.2 ± 0.095 mg 槲皮素当量/克干提取物（mg QE/g DE）。另一方面，通过测定清除自由基 DPPH 和 -胡萝卜素/亚油酸模型系统的能力，来量化叶提取物的抗氧化活性，其值分别达到 0.0027 ± 0.002 mg/mL 和 0.128 ± 0.04 mg/mL。关于抗糖尿病活性，基于对胰腺 -淀粉酶活性的抑制作用，观察到约 68.20%的显著抑制作用，IC 值为 0.266 mg/mL。这一发现与提取物主要酚类化合物的分子对接研究一致，其中观察到对 -淀粉酶具有显著的结合亲和力，主要化合物儿茶素、槲皮素 3-葡萄糖苷和没食子酸的结合亲和力值分别为-7.631（kcal/mol）、-6.818（kcal/mol）和-5.517（kcal/mol）。
