Mikhaĭlov A T, Gorgoliuk N A, Mukhoian M M
Ontogenez. 1993 Mar-Apr;24(2):24-32.
The data are presented about isolation from the brain of 7.5-day chick embryos of a factor capable of neuralizing effect on the early gastrula ectoderm of the grass frog Rana temporaria L. Earlier this factor was defined as embryonic brain-derived neuralizing factor (EBDNF) (Mikhailov, Gorgoliuk, 1989). The isolation procedure includes (1) extraction with deionized water at pH 9.0; (2) ion exchange chromatography on a column with DEAE-adsorbent at pII 8.0; (3) affinity chromatography on Heparin-Ultragel column. EBDNF-containing fraction is eluted from the Heparin-Ultragel column with 250 mM NaCl as a separate peak. Four bands are observed on SDS-electrophoregrams of this fraction, two more prominent ones having the molecular weight of 43 and 63 kDa. The yield of EBDNF-containing fraction is about 0.01-0.02% of the wet weight of the initial brain tissue.
本文呈现了从7.5日龄鸡胚大脑中分离出一种因子的数据，该因子对欧洲林蛙早期原肠胚外胚层具有神经化作用。此前该因子被定义为胚胎脑源性神经化因子（EBDNF）（米哈伊洛夫、戈尔戈柳克，1989年）。分离过程包括：（1）在pH 9.0条件下用去离子水提取；（2）在pH 8.0条件下于装有DEAE吸附剂的柱上进行离子交换色谱；（3）在肝素-超凝胶柱上进行亲和色谱。含EBDNF的组分以250 mM NaCl从肝素-超凝胶柱上洗脱下来，形成一个单独的峰。该组分的SDS电泳图谱上观察到四条带，其中两条较明显的带分子量分别为43 kDa和63 kDa。含EBDNF组分的产量约为初始脑组织湿重的0.01 - 0.02%。
