Lu Yangyang, Yin Xuechi, Li Mei, Ma Wanlu, Du Sining, Wang Zixuan, Qiu Nannan, Zhao Xubo
College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, Shaanxi, China.
NHC Key Laboratory of Food Safety Risk Assessment, Chinese Academy of Medical Science (2019RU014), China National Center for Food Safety Risk Assessment, Beijing, 100021, China.
Talanta. 2025 May 1;286:127487. doi: 10.1016/j.talanta.2024.127487. Epub 2024 Dec 29.
In the study, an eccentric heterogeneous core-shell nanomaterial Au@CuSe was simply and rapidly synthesized. This novel nano-structure exhibits superior colorimetric intensity, enhanced antibody coupling efficiency, and strong broadband absorption across the visible to near-infrared spectrum, with a photothermal conversion efficiency of 59.40%. As proof of concept, we applied Au@CuSe as a signal amplification marker in LFIA for the rapid detection of zearalenone (ZEN), enabling dual-mode quantitative detection via colorimetric and photothermal signals (CM/PT). The Au@CuSe-LFIA demonstrated a cut-off value of 10 ng/mL in colorimetric mode and 5 ng/mL in photothermal mode, representing a two fold increase in sensitivity compared to traditional AuNPs-based LFIAs. The outstanding colorimetric signal and prominent photothermal signal make the proposed Au@CuSe nanomaterial show better application prospects in CM/PT mode detection in LFIA, providing broad application prospects for the broad-spectrum detection of mycotoxins in food in the future.
在该研究中，一种偏心异质核壳纳米材料Au@CuSe被简单快速地合成出来。这种新型纳米结构表现出优异的比色强度、增强的抗体偶联效率以及在可见光到近红外光谱范围内的强宽带吸收，光热转换效率为59.40%。作为概念验证，我们将Au@CuSe用作侧向流动免疫分析（LFIA）中用于快速检测玉米赤霉烯酮（ZEN）的信号放大标记物，实现了通过比色和光热信号（CM/PT）进行双模式定量检测。Au@CuSe-LFIA在比色模式下的截断值为10 ng/mL，在光热模式下为5 ng/mL，与传统基于金纳米颗粒（AuNPs）的LFIA相比，灵敏度提高了两倍。出色的比色信号和显著的光热信号使得所提出的Au@CuSe纳米材料在LFIA的CM/PT模式检测中显示出更好的应用前景，为未来食品中霉菌毒素的广谱检测提供了广阔的应用前景。
