Jiang Donglei, Feng Zeng, Jiang Hui, Xiang Xinyue, Wang Lifeng
College of Food Science and Engineering, Collaborative Innovation Center for Modern Grain Circulation and Safety, Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics, Nanjing, Jiangsu, 210023, PR China.
Nanjing Institute for Food and Drug Control, Nanjing, Jiangsu, 211198, PR China.
Biosens Bioelectron. 2025 Mar 1;271:117103. doi: 10.1016/j.bios.2024.117103. Epub 2024 Dec 25.
An innovative integrated three-dimensional (3D) bioprinted gastric microtissue electrochemical biosensor was developed in this study for the detection of allergen ovalbumin (OVA). In this system, OVA triggers the release of histamine from gastric microtissue, which then undergoes a redox reaction on the electrode surface, leading to an increase in the peak current. Gelatin methacrylate hydrogel serves as a scaffold for the 3D culture of RBL-2H3 and PC-12 cells for partially restoring allergic reactions in the human body in vitro. Furthermore, gold nanoparticle-modified anodized aluminum oxide sieves macromolecular substances and facilitates sensor nano-analysis. Composites of cerium-based organometallic framework, MnO, and gold nanoparticles significantly enhanced the sensitivity of the screen-printed carbon electrode. Under optimal experimental conditions, the detection limit for OVA was 0.042 μg/mL, with a linear range of 0.1-10.0 μg/mL. The fabricated sensor demonstrated high sensitivity, reliability, and simplicity, showcasing its broad potential for allergen detection applications.
本研究开发了一种创新的集成三维（3D）生物打印胃微组织电化学生物传感器，用于检测过敏原卵清蛋白（OVA）。在该系统中，OVA触发胃微组织释放组胺，组胺随后在电极表面发生氧化还原反应，导致峰值电流增加。甲基丙烯酸明胶水凝胶作为RBL-2H3和PC-12细胞3D培养的支架，用于在体外部分恢复人体的过敏反应。此外，金纳米颗粒修饰的阳极氧化铝筛可筛分大分子物质，并有助于传感器的纳米分析。铈基金属有机框架、MnO和金纳米颗粒的复合材料显著提高了丝网印刷碳电极的灵敏度。在最佳实验条件下，OVA的检测限为0.042μg/mL，线性范围为0.1-10.0μg/mL。所制备的传感器具有高灵敏度、可靠性和简易性，在过敏原检测应用中具有广阔的潜力。
