College of Food Science and Engineering, Collaborative Innovation Center for Modern Grain Circulation and Safety, Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics, Nanjing, Jiangsu 210023, PR China.
Nanjing Institute for Food and Drug Control, Nanjing, Jiangsu 211198, PR China.
Food Chem. 2024 Jul 1;445:138799. doi: 10.1016/j.foodchem.2024.138799. Epub 2024 Feb 18.
A novel 3D bio-printing vascular microtissue biosensor was developed to detect fish parvalbumin quickly. The graphite rod electrode was modified with gold and copper organic framework (Cu-MOF) to improve the sensor properties. Polydopamine-modified multi-wall carbon nanotubes (PDA-MWCNT) were mixed with gelatin methacryloyl (GelMA) to prepare a conductive hydrogel. The conductive hydrogel was mixed with mast cells and endothelial cells to produce a bio-ink for 3D bioprinting. High throughput and standardized preparation of vascular microtissue was performed by stereolithography 3D bioprinting. The vascular microtissue was immobilized on the modified electrode to construct the microtissue sensor. The biosensor's peak current was positively correlated with the fish parvalbumin concentration, and the detection linear concentration range was 0.1 ∼ 2.5 μg/mL. The standard curve equation was I = 31.30 + 5.46 C, the correlation coefficient R was 0.990 (n = 5), and the detection limit was 0.065 μg/mL. These indicated a biomimetic microtissue sensor detecting fish parvalbumin has been successfully constructed.
一种新型的 3D 生物打印血管微组织生物传感器被开发出来，用于快速检测鱼类副肌球蛋白。石墨棒电极经过金和铜有机框架（Cu-MOF）修饰，以提高传感器性能。聚多巴胺修饰的多壁碳纳米管（PDA-MWCNT）与明胶甲基丙烯酰（GelMA）混合，制备导电水凝胶。导电水凝胶与肥大细胞和内皮细胞混合，用于 3D 生物打印制备生物墨水。通过立体光刻 3D 生物打印实现血管微组织的高通量和标准化制备。将血管微组织固定在修饰电极上，构建微组织传感器。该生物传感器的峰电流与鱼类副肌球蛋白浓度呈正相关，检测线性浓度范围为 0.1∼2.5μg/mL。标准曲线方程为 I=31.30+5.46C，相关系数 R 为 0.990（n=5），检测限为 0.065μg/mL。这些结果表明，已经成功构建了用于检测鱼类副肌球蛋白的仿生微组织传感器。
