Krinke G, Naylor D C, Skorpil V
J Neuropathol Exp Neurol. 1985 Mar;44(2):117-29.
The early effects of high toxic doses of vitamin B6 (pyridoxine) on the peripheral sensory neurons were studied in laboratory rats. The animals were treated with 600 mg/kg of pyridoxine hydrochloride by intraperitoneal injection twice daily. Thereafter they were killed by perfusion-fixation at periods ranging from one to 14 days and the tissues were examined by light and electron microscopy. The primary change consisted of the formation of swollen membranous profiles in both the axon hillock and the initial axonal segment of the large dorsal root cytons. This change occurred within 24 hours of exposure, and was followed by an axonal reaction of the nerve cell bodies and by secondary degeneration of their processes. These findings identify the probable target site for pyridoxine toxicity, and establish a simple animal model for studying not only sensory denervation, but also the axonal reaction and secondary degeneration.
在实验大鼠中研究了高毒性剂量维生素B6（吡哆醇）对周围感觉神经元的早期影响。通过腹腔注射，每天给动物两次600mg/kg盐酸吡哆醇。此后，在1至14天的不同时间段，通过灌注固定法处死动物，并通过光镜和电镜检查组织。主要变化包括大的背根神经节细胞的轴丘和轴突起始段出现肿胀的膜性轮廓。这种变化在暴露后24小时内发生，随后是神经细胞体的轴突反应及其突起的继发性变性。这些发现确定了吡哆醇毒性的可能靶点，并建立了一个简单的动物模型，不仅用于研究感觉神经去神经支配，还用于研究轴突反应和继发性变性。
