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落花生单酰甘油脂肪酶AhMAGL3b参与脂质代谢。

Arachis hypogaea monoacylglycerol lipase AhMAGL3b participates in lipid metabolism.

作者信息

Zhan Yihua, Wang Jing, Zhao Xuan, Zheng Zhifu, Gan Yi

机构信息

College of Advanced Agricultural Sciences, Zhejiang A & F University, Hangzhou, 311300, China.

出版信息

BMC Plant Biol. 2024 Dec 30;24(1):1278. doi: 10.1186/s12870-024-06017-0.

DOI:10.1186/s12870-024-06017-0
PMID:39736532
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11687083/
Abstract

BACKGROUND

Monoacylglycerol lipase (MAGL) belongs to the serine hydrolase family; it catalyzes MAG to produce glycerol and free fatty acids (FFAs), which is the final step in triacylglycerol (TAG) hydrolysis. The effects of MAGL on comprehensive lipid metabolism and plant growth and development have not been elucidated, especially in Arachis hypogaea, an important oil crop.

RESULTS

Herein, AhMAGL3b encoding a protein with both hydrolase and acyltransferase regions, a member of MAGL gene family, was cloned and overexpressed in Arabidopsis thaliana. A total of 9 homozygous T generation transgenic lines were obtained. Compared with wild type (WT), overexpression (OE) of AhMAGL3b had no obvious growth inhibition by investigation of agronomic traits, including growth and photosynthetic parameters. The leaf fatty acid (FA) content was increased by 12.1-27.4% in AhMAGL3b-OE lines, while seed oil content was decreased by 10.7-17.3%. Furthermore, the overexpression of AhMAGL3b resulted in higher soluble sugar and starch content, and lower total soluble protein content in both leaves and seeds. Additionally, during seed germination, AhMAGL3b-OE seeds were more dormant than that of WT and the sensitivity to abscisic acid (ABA) treatment was decreased.

CONCLUSIONS

Taken together, our results indicate that AhMAGL3b is involved in homeostasis among carbohydrates, lipids and protein in A. hypogaea.

摘要

背景

单酰甘油脂肪酶（MAGL）属于丝氨酸水解酶家族；它催化单酰甘油（MAG）生成甘油和游离脂肪酸（FFA），这是三酰甘油（TAG）水解的最后一步。MAGL对综合脂质代谢以及植物生长发育的影响尚未阐明，尤其是在重要油料作物花生中。

结果

在此，克隆了编码具有水解酶和酰基转移酶区域的蛋白质的AhMAGL3b，它是MAGL基因家族的成员，并在拟南芥中过表达。共获得9个纯合的T代转基因株系。通过对包括生长和光合参数在内的农艺性状进行调查，发现与野生型（WT）相比，AhMAGL3b的过表达（OE）没有明显的生长抑制。AhMAGL3b过表达株系的叶片脂肪酸（FA）含量增加了12.1 - 27.4%，而种子油含量降低了10.7 - 17.3%。此外，AhMAGL3b的过表达导致叶片和种子中的可溶性糖和淀粉含量升高，总可溶性蛋白含量降低。另外，在种子萌发期间，AhMAGL3b过表达的种子比WT种子更休眠，并且对脱落酸（ABA）处理的敏感性降低。

结论

综上所述，我们的结果表明AhMAGL3b参与了花生中碳水化合物、脂质和蛋白质之间的稳态。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ede8/11687083/d5ffffb354fb/12870_2024_6017_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ede8/11687083/48020439ee57/12870_2024_6017_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ede8/11687083/a60b8bc2add9/12870_2024_6017_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ede8/11687083/cf5d0f0a03fe/12870_2024_6017_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ede8/11687083/a438091197ac/12870_2024_6017_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ede8/11687083/939a9468ef2c/12870_2024_6017_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ede8/11687083/d5ffffb354fb/12870_2024_6017_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ede8/11687083/48020439ee57/12870_2024_6017_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ede8/11687083/a60b8bc2add9/12870_2024_6017_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ede8/11687083/cf5d0f0a03fe/12870_2024_6017_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ede8/11687083/a438091197ac/12870_2024_6017_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ede8/11687083/939a9468ef2c/12870_2024_6017_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ede8/11687083/d5ffffb354fb/12870_2024_6017_Fig6_HTML.jpg

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