Zhang Zhihao, He Ting, Gu Hongwen, Zhao Yuanhang, Tang Shilei, Han Kangen, Hu Yin, Wang Hongwei, Yu Hailong
General Hospital of Northern Theater Command, Shenyang, Liaoning Province, 110000, China.
Xi'an Key Laboratory of Stem Cell and Regenerative Medicine, Institute of Medical Research, Northwestern Polytechnical University, Xi'an, China.
BMC Mol Cell Biol. 2024 Dec 30;25(1):28. doi: 10.1186/s12860-024-00519-3.
In recent years, chondrocytes have been found to contain hemoglobin, which might be an alternative strategy for adapting to the hypoxic environment, while the potential mechanisms of that is still unclear. Here, we report the expression characteristics and potential associated pathways of hemoglobin in chondrocytes using single-cell RNA sequencing (scRNA-seq). We downloaded data of normal people and patients with osteoarthritis (OA) from the Gene Expression Omnibus (GEO) database and cells are unbiased clustered based on gene expression pattern. We determined the expression levels of hemoglobin in various chondrocyte subpopulations. Meanwhile, we further explored the difference in the enriched signaling pathways and the cell-cell interaction in chondrocytes of the hemoglobin high-expression and low-expression groups. Specifically, we found that SPP1 was closely associated with the expression of hemoglobin in OA progression. Our findings provide new insights into the distribution characteristics of hemoglobin in chondrocytes and provide potential clues to the underlying role of hemoglobin in OA and the mechanisms related to that, providing potential new ideas for the treatment of OA.
近年来，人们发现软骨细胞中含有血红蛋白，这可能是适应缺氧环境的一种替代策略，但其潜在机制仍不清楚。在此，我们使用单细胞RNA测序（scRNA-seq）报告了软骨细胞中血红蛋白的表达特征和潜在相关途径。我们从基因表达综合数据库（GEO）下载了正常人和骨关节炎（OA）患者的数据，并根据基因表达模式对细胞进行无偏聚类。我们确定了各种软骨细胞亚群中血红蛋白的表达水平。同时，我们进一步探讨了血红蛋白高表达组和低表达组软骨细胞中富集信号通路和细胞间相互作用的差异。具体而言，我们发现SPP1与OA进展过程中血红蛋白的表达密切相关。我们的研究结果为血红蛋白在软骨细胞中的分布特征提供了新的见解，并为血红蛋白在OA中的潜在作用及其相关机制提供了潜在线索，为OA的治疗提供了潜在的新思路。
