Ogedengbe O O, Imoyin Omene O, Adekeye A O, Ajiboye B O, Adeeyo O A
Department of Anatomy, Faculty of Basic Medical Sciences, Federal University Oye-Ekiti, Oye-Ekiti, Ekiti State, Nigeria.
Department of Anatomy, College of Medicine and Health Sciences, Afe Babalola University, Ado Ekiti, Ekiti State, Nigeria.
Arch Razi Inst. 2024 Jun 30;79(3):565-574. doi: 10.32592/ARI.2024.79.3.565. eCollection 2024 Jun.
Prolonged use of antiretroviral agents has been clearly associated with nephrotoxicity, suggesting deterioration of renal function in patients receiving Highly Active Antiretroviral Therapy (HAART). The present study was designed to investigate the therapeutic efficacy of resveratrol (RV) in the treatment toxins-induced renal impairment. Twenty-four adult male Wistar rats weighing 70-90 g were divided into four groups and subjected to the following treatments: Control A (distilled water), B (HAART), C (RV-2.5 mg/kg), D (RV- 2.5 mg/kg) + HAART. Assessment included renal histological examination; renal function indicators such as serum creatinine and blood urea nitrogen; serum electrolyte levels including sodium, chloride, potassium, bicarbonate; and oxidative stress biomarkers such as malondialdehyde, catalase and glutathione and superoxide dismutase. Adverse effects of HAART include adverse histological changes, such as tubular atrophy, vacuolization, tubular granular degeneration and glomerular capillaries abnormalities. Compared to the other treatment cohorts, serum creatinine, blood urea nitrogen (BUN), sodium, chloride and malondialdehyde (MDA) levels were significantly increased, while antioxidant enzyme activities such as catalase (CAT) and superoxide dismutase (SOD) and glutathione (GSH) levels were notably decreased. Renal structure remained largely unchanged after RV administration, with some recovery in histological abnormalities. Visible improvements, including reduced inflammation, reduced necrosis, reduced vacuolization and improved tubule and glomerular configuration, were also observed. In addition, RV notably increased antioxidant enzyme levels (SOD, CAT, and GSH) and decreased BUN, serum creatinine and MDA levels. RV helped mitigate HAART-induced structural abnormalities and renal dysfunction, while improving renal morphology. However, further investigation of these mechanisms is needed.
长期使用抗逆转录病毒药物已被明确证实与肾毒性有关，这表明接受高效抗逆转录病毒疗法（HAART）的患者肾功能会恶化。本研究旨在探讨白藜芦醇（RV）对毒素诱导的肾损伤的治疗效果。将24只体重70 - 90克的成年雄性Wistar大鼠分为四组，并进行以下处理：对照组A（蒸馏水）、B（HAART）、C（RV - 2.5毫克/千克）、D（RV - 2.5毫克/千克）+ HAART。评估内容包括肾脏组织学检查；肾功能指标，如血清肌酐和血尿素氮；血清电解质水平，包括钠、氯、钾、碳酸氢根；以及氧化应激生物标志物，如丙二醛、过氧化氢酶、谷胱甘肽和超氧化物歧化酶。HAART的不良反应包括不良组织学变化，如肾小管萎缩、空泡化、肾小管颗粒变性和肾小球毛细血管异常。与其他治疗组相比，血清肌酐、血尿素氮（BUN）、钠、氯和丙二醛（MDA）水平显著升高，而过氧化氢酶（CAT）、超氧化物歧化酶（SOD）等抗氧化酶活性以及谷胱甘肽（GSH）水平则显著降低。给予RV后肾脏结构基本保持不变，组织学异常有一定程度的恢复。还观察到明显改善，包括炎症减轻、坏死减少、空泡化减少以及肾小管和肾小球结构改善。此外，RV显著提高了抗氧化酶水平（SOD、CAT和GSH），并降低了BUN、血清肌酐和MDA水平。RV有助于减轻HAART诱导的结构异常和肾功能障碍，同时改善肾脏形态。然而，需要对这些机制进行进一步研究。
