Paczkowska-Walendowska Magdalena, Miklaszewski Andrzej, Michniak-Kohn Bożena, Cielecka-Piontek Judyta
Department of Pharmacognosy and Biomaterials, Poznan University of Medical Sciences, Rokietnicka 3, 60-806 Poznan, Poland.
Faculty of Mechanical Engineering and Management, Institute of Materials Science and Engineering, Poznan University of Technology, 60-965 Poznan, Poland.
Antioxidants (Basel). 2023 Sep 18;12(9):1777. doi: 10.3390/antiox12091777.
Despite the wide pharmacological action of polyphenols, their usefulness is limited due to their low oral bioavailability, which is due to their low solubility and rapid first-pass metabolism. Red vine leaf extract is an herbal medicine containing several polyphenols, with resveratrol and polydatin as the main compounds exhibiting antioxidant and anti-inflammatory properties. In the first stage of the work, using the Design of Experiment (DoE) approach, the red vine leaf extract (50% methanol, temperature 70 °C, and three cycles per 60 min) was obtained, which showed optimal antioxidant and anti-inflammatory properties. In order to circumvent the above-described limitations and use innovative technology, electrospun nanofibers containing the red vine leaf extract, polyvinylpyrrolidone (PVP), and hydroxypropyl-β-cyclodextrin (HPβCD) were first developed. The optimization of the process involved the time of system mixing prior to electrospinning, the mixture flow rate, and the rotation speed of the collector. Dissolution studies of nanofibers showed improved resveratrol release from the nanofibers (over five-fold). Additionally, a PAMPA-GIT assay confirmed significantly better buccal penetration of resveratrol from this nanofiber combination (over ten-fold). The proposed strategy for electrospun nanofibers with the red vine leaf extract is an innovative approach to better use the synergy of the biological action of active compounds present in extracts that are beneficial for the development of nutraceuticals.
尽管多酚具有广泛的药理作用，但其口服生物利用度低，限制了其效用，这是由于其溶解度低和首过代谢快所致。红葡萄叶提取物是一种含有多种多酚的草药，白藜芦醇和白藜芦醇苷是主要化合物，具有抗氧化和抗炎特性。在工作的第一阶段，采用实验设计（DoE）方法，获得了红葡萄叶提取物（50%甲醇，温度70°C，每60分钟三个循环），其表现出最佳的抗氧化和抗炎特性。为了克服上述局限性并采用创新技术，首先开发了含有红葡萄叶提取物、聚乙烯吡咯烷酮（PVP）和羟丙基-β-环糊精（HPβCD）的电纺纳米纤维。该工艺的优化涉及电纺前系统混合时间、混合物流速和收集器转速。纳米纤维的溶出研究表明，白藜芦醇从纳米纤维中的释放有所改善（超过五倍）。此外，PAMPA-GIT试验证实，这种纳米纤维组合中白藜芦醇的颊部渗透明显更好（超过十倍）。所提出的含有红葡萄叶提取物的电纺纳米纤维策略是一种创新方法，能够更好地利用提取物中活性化合物生物作用的协同效应，这对营养保健品的开发有益。
Zotero 插件
