Theja Letitia Amanda, Kusnoto Kusnoto, Tacharina Martia Rani, Suwanti Lucia Tri, Mufasirin Mufasirin, Hastutiek Poedji, Aryaloka Suhita, Khairullah Aswin Rafif, Moses Ikechukwu Benjamin, Raissa Ricadonna, Mulyaningrum Putri Wahyu, Yanestria Sheila Marty, Riwu Katty Hendriana Priscilia
Profession Program in Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
Division of Veterinary Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
Open Vet J. 2024 Nov;14(11):2989-2994. doi: 10.5455/OVJ.2024.v14.i11.27. Epub 2024 Nov 30.
is a known cause of a zoonotic infectious illness called toxocariasis. Parathenic hosts are important as they can transmit larvae 2 (L) through direct transmission. Scanning electron microscope (SEM) techniques are needed to provide a three-dimensional image of each stage of larvae.
The aim of this study was to determine the morphology of L and L in parathenic host tissue for etiological diagnosis using SEM.
Mice were used as suitable paratenic hosts for this experiment. A total of 786 embryonated eggs (16 eggs/gram body weight) containing L were inoculated into pregnant mice at day 11-13 of its gestation period. After delivery, L was transmitted to the off-spring. After 14 days, L was collected from mice, and L was collected from its off-spring. Data were analyzed descriptively based on ultrastructure examination using SEM.
SEM examination results indicate that the size of L is smaller than L. Results also showed differences between L and L based on middle and posterior observations. In the middle of the larval body, the number of L body rings was observed to be narrower and more than that of L. In addition, the distance between L body rings was much larger than that of L. Posteriorly, the tail tip of L was more curved than L.
Ultrastructural examination using SEM has the ability to show differences in L and L body rings of by observing the middle and posterior parts of its larvae.
是一种名为弓蛔虫病的人畜共患传染病的已知病因。转续宿主很重要，因为它们可以通过直接传播来传播第二期幼虫（L2）。需要扫描电子显微镜（SEM）技术来提供幼虫各个阶段的三维图像。
本研究的目的是使用SEM确定转续宿主组织中L2和L3的形态，用于病因诊断。
将小鼠用作本实验合适的转续宿主。在妊娠第11 - 13天，将总共786个含L2的受精虫卵（16个虫卵/克体重）接种到怀孕小鼠体内。分娩后，L2传播给后代。14天后，从小鼠体内收集L2，并从其后代体内收集L3。基于使用SEM的超微结构检查进行描述性数据分析。
SEM检查结果表明，L2的尺寸小于L3。结果还显示，基于中部和尾部观察，L2和L3之间存在差异。在幼虫身体中部，观察到L2的体环数量更窄且比L3更多。此外，L2体环之间的距离比L3大得多。在尾部，L2的尾尖比L3更弯曲。
使用SEM的超微结构检查能够通过观察幼虫的中部和尾部来显示L2和L3体环的差异。
