Ultrastructural morphology of second and third-stage larvae of inside paratenic host tissue.

BACKGROUND

is a known cause of a zoonotic infectious illness called toxocariasis. Parathenic hosts are important as they can transmit larvae 2 (L) through direct transmission. Scanning electron microscope (SEM) techniques are needed to provide a three-dimensional image of each stage of larvae.

AIM

The aim of this study was to determine the morphology of L and L in parathenic host tissue for etiological diagnosis using SEM.

METHODS

Mice were used as suitable paratenic hosts for this experiment. A total of 786 embryonated eggs (16 eggs/gram body weight) containing L were inoculated into pregnant mice at day 11-13 of its gestation period. After delivery, L was transmitted to the off-spring. After 14 days, L was collected from mice, and L was collected from its off-spring. Data were analyzed descriptively based on ultrastructure examination using SEM.

RESULTS

SEM examination results indicate that the size of L is smaller than L. Results also showed differences between L and L based on middle and posterior observations. In the middle of the larval body, the number of L body rings was observed to be narrower and more than that of L. In addition, the distance between L body rings was much larger than that of L. Posteriorly, the tail tip of L was more curved than L.

CONCLUSION

Ultrastructural examination using SEM has the ability to show differences in L and L body rings of by observing the middle and posterior parts of its larvae.