Ramadhani Safira, Khairullah Aswin Rafif, Effendi Mustofa Helmi, Sukmanadi Mohammad, Tyasningsih Wiwiek, Millannia Saumi Kirey, Afnani Daniah Ashri, Moses Ikechukwu Benjamin, Farizqi M Thoriq Ihza, Kaben Sergius Erikson
Profession Program of Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
Research Center for Veterinary Science, National Research and Innovation Agency (BRIN), Bogor, Indonesia.
Open Vet J. 2024 Nov;14(11):3026-3036. doi: 10.5455/OVJ.2024.v14.i11.31. Epub 2024 Nov 30.
The most susceptible group of people to spread methicillin-resistant (MRSA) among domestic cats is their owners' relatives.
Considering the aforementioned, research at the Surabaya City Animal Hospital is necessary to determine whether the A gene may be detected in cat nasal swabs.
Samples were taken using a sterile cotton swab, and the transport medium was buffered peptone water. Standard operating procedures in microbiology were then employed to identify the samples. Using five distinct antibiotic discs, the Kirby-Bauer diffusion method was used to determine the antibiotic resistance profile of . All cefoxitin-resistant isolates were tested for MRSA and then the A gene was detected.
Based on morphological culture features, Gram staining, and biochemical assays, 64 samples (64%) of the 100 isolated cat nose swab samples were positive for , according to the results of the biochemical tests. The profile of antibiotic resistance developed from the results of the resistance test to antibiotics revealed that five (7.81%) of the 64 isolates were confirmed to be multidrug resistant. Out of the 6 MRSA isolates analyzed, the A gene was detected in 5 isolates.
Based on the findings of this study, a thorough assessment of the impact of using antibiotics to prevent the spread of MRSA in veterinary hospitals on the general public's health is required in light of the detection of MRSA isolates bearing the A gene in the Surabaya veterinary hospital.
在家养猫中，最易传播耐甲氧西林金黄色葡萄球菌（MRSA）的人群是其主人的亲属。
鉴于上述情况，泗水市动物医院有必要开展研究，以确定是否能在猫鼻拭子中检测到A基因。
使用无菌棉拭子采集样本，运输培养基为缓冲蛋白胨水。然后采用微生物学标准操作程序对样本进行鉴定。使用五种不同的抗生素药敏纸片，采用 Kirby-Bauer 扩散法测定……的抗生素耐药谱。对所有耐头孢西丁的……分离株进行 MRSA 检测，然后检测 A 基因。
根据生化试验结果，在 100 份分离的猫鼻拭子样本中，64 份样本（64%）根据生化试验结果，基于形态学培养特征、革兰氏染色和生化分析，对……呈阳性。从对……的抗生素耐药性测试结果得出的抗生素耐药谱显示，64 株……分离株中有 5 株（7.81%）被确认为多重耐药。在分析的 6 株 MRSA 分离株中，有 5 株检测到 A 基因。
根据本研究结果，鉴于泗水市兽医医院检测到携带 A 基因的 MRSA 分离株，需要全面评估在兽医医院使用抗生素预防 MRSA 传播对公众健康的影响。
