Fatimah Nurmawati, Sudjarwo Sri Agus, Mustika Arifa, Suharjono Suharjono, Nugraha Jusak, Notobroto Hari Basuki, Kurnijasanti Rochmah, I'tishom Reny, Riawan Wibi, Rahniayu Alphania
Doctoral Program of Medical Science, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
Department of Anatomy, Histology and Pharmacology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
Open Vet J. 2024 Nov;14(11):2970-2979. doi: 10.5455/OVJ.2024.v14.i11.25. Epub 2024 Nov 30.
Hyperglycemia is a condition in which blood sugar levels increase excessively due to a variety of factors, one of which is the body's inability to regulate insulin properly. Diabetes closely relates to this condition, which significantly contributes to premature death and disability. Long-term diabetes treatment accompanied by a strict diet provides real results in controlling blood glucose levels but can cause side effects. Therefore, it is necessary to conduct research on the potential for new drug sources with minimal side effects. Traditional medicine empirically uses the plant .
This study was conducted to determine the effect of on hyperglycemic model rat and to further determine the mechanism of its active compounds .
The experimental animals were divided into 5 groups: 1 normal group, 1 group induced with Streptozotocin (STZ) 50 mg/kgBW to treat hyperglycemia, and 3 other groups induced with STZ 50 mg/kgBW and given 70% ethanol extract of leaves (EEPa) in different doses. Each group was measured for B-cell lymphoma 2 (Bcl2) expression and apoptosis. We also carried out identification of the isoarborinol acetate and myricitrin compounds contained in EEPa against alpha-glucosidase and caspase-3.
It was found that giving STZ to rat can cause hyperglycemia. This was shown by measuring fasting blood glucose in rat and then measuring Bcl2 as an antiapoptotic agent. Bcl2 levels rose compared to the hyperglycemic control group and can lower apoptosis expression in heart cells of hyperglycemic model rat with an optimal dose of 90 mg/kgBW. In addition, the results showed that isoarborinol acetate and myricitrin compounds have inhibition of alpha-glucosidase and caspase-3.
It can be concluded that EEPa is one of the alternative choices to overcome hyperglycemia.
高血糖是一种由于多种因素导致血糖水平过度升高的病症，其中一个因素是身体无法正常调节胰岛素。糖尿病与这种病症密切相关，它是导致过早死亡和残疾的重要原因。长期糖尿病治疗并配合严格饮食确实能有效控制血糖水平，但可能会产生副作用。因此，有必要研究副作用最小的新药源潜力。传统医学经验性地使用该植物。
本研究旨在确定[植物名称]对高血糖模型大鼠的影响，并进一步确定其活性成分的作用机制。
将实验动物分为5组：1个正常组，1个用链脲佐菌素（STZ）50 mg/kg体重诱导以治疗高血糖的组，以及另外3个用STZ 50 mg/kg体重诱导并给予不同剂量的[植物名称]叶70%乙醇提取物（EEPa）的组。每组均检测B细胞淋巴瘤2（Bcl2）表达和细胞凋亡情况。我们还对EEPa中含有的乙酸异乔木醇酯和杨梅苷化合物针对α-葡萄糖苷酶和半胱天冬酶-3进行了鉴定。
发现给大鼠注射STZ会导致高血糖。通过测量大鼠空腹血糖以及随后测量作为抗凋亡剂的Bcl2得以证明。与高血糖对照组相比，Bcl2水平升高，且在最佳剂量为90 mg/kg体重时可降低高血糖模型大鼠心脏细胞中的凋亡表达。此外，结果表明乙酸异乔木醇酯和杨梅苷化合物对α-葡萄糖苷酶和半胱天冬酶-3有抑制作用。
可以得出结论，EEPa是克服高血糖的替代选择之一。
