Metabolic and Biomolecular Engineering National Research Laboratory and Systems Metabolic Engineering and Systems Healthcare Cross-Generation Collaborative Laboratory, Department of Chemical and Biomolecular Engineering (BK21 four), Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, the Republic of Korea; KAIST Institute for the BioCentury and KAIST Institute for Artificial Intelligence, KAIST, Daejeon 34141, the Republic of Korea; BioProcess Engineering Research Center and BioInformatics Research Center, KAIST, 34141 Daejeon, the Republic of Korea.
Metabolic and Biomolecular Engineering National Research Laboratory and Systems Metabolic Engineering and Systems Healthcare Cross-Generation Collaborative Laboratory, Department of Chemical and Biomolecular Engineering (BK21 four), Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, the Republic of Korea; KAIST Institute for the BioCentury and KAIST Institute for Artificial Intelligence, KAIST, Daejeon 34141, the Republic of Korea.
Curr Opin Biotechnol. 2023 Dec;84:103004. doi: 10.1016/j.copbio.2023.103004. Epub 2023 Sep 29.
Systems metabolic engineering, which integrates metabolic engineering with systems biology, synthetic biology, and evolutionary engineering, has revolutionized the sustainable production of fuels and materials through the creation of efficient microbial cell factories. Recent advancements in systems metabolic engineering targeting different biological components of the host cell have enabled the creation of highly productive microbial cell factories. This article provides a review of the recent tools and strategies used for enzyme-, genetic module-, pathway-, flux-, genome-, and cell-level engineering, supported by illustrative examples. Furthermore, we highlight recent trends in systems metabolic engineering, which involve the application of multiple tools discussed in this review. Finally, the paper addresses the challenges and perspectives of transitioning academic-level metabolic engineering studies to commercial-scale production.
系统代谢工程将代谢工程与系统生物学、合成生物学和进化工程相结合，通过创建高效的微生物细胞工厂，彻底改变了燃料和材料的可持续生产。针对宿主细胞不同生物成分的系统代谢工程的最新进展，使我们能够创建具有高生产力的微生物细胞工厂。本文综述了酶、遗传模块、途径、通量、基因组和细胞水平工程中使用的最新工具和策略，并提供了说明性实例。此外，我们还强调了系统代谢工程的最新趋势，其中涉及到本文讨论的多种工具的应用。最后，本文讨论了将学术水平的代谢工程研究转化为商业规模生产所面临的挑战和展望。
